Characterization and chromosomal assignment of yeast artificial chromosomes containing human 3p13-p21-specific sequence tagged sites

Susanne C. Michaelis, Walter Bardenheuer, Andreas Lux, Alexander Schramm, Anja Gockel, Reiner Siebert, Christoph Willers, Katja Schmidtke, Birgit Todt, Annemarie H. van der Hout, Charles H.C.M. Buys, Amanda C. Heppell-Parton, Pamela H. Rabbitts, Sylvia Ungar, David Smith, Denis LePaslier, Daniel Cohen, Bertram Opalka, Jochen Schütte

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, andother neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a chromosome 3p14 microdissection library. STSs were used for isolating yeast artificial chromosome (YAC) clones from the Centre d'Etude du Polymorphisme Humain (CEPH) YAC libraries. Thirty-eight YACs were assembled into a contig approximately 2.5 Mb in size spanning the t(3;8) and t(3;6) translocation breakpoints associated with hereditary renal cell carcinoma and hematologic malignancies, respectively. Chromosomal localization and chimeric status of 126 YACs was analyzed by fluorescence in situ hybridization (FISH). The order of 17 YACs determined by double-color FISH was in agreement with the STS-based arrangement of the YAC-contig.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalCancer Genetics and Cytogenetics
Volume81
Issue number1
DOIs
StatePublished - May 1995

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Michaelis, S. C., Bardenheuer, W., Lux, A., Schramm, A., Gockel, A., Siebert, R., Willers, C., Schmidtke, K., Todt, B., van der Hout, A. H., Buys, C. H. C. M., Heppell-Parton, A. C., Rabbitts, P. H., Ungar, S., Smith, D., LePaslier, D., Cohen, D., Opalka, B., & Schütte, J. (1995). Characterization and chromosomal assignment of yeast artificial chromosomes containing human 3p13-p21-specific sequence tagged sites. Cancer Genetics and Cytogenetics, 81(1), 1-12. https://doi.org/10.1016/0165-4608(94)00208-8