Characteristics of tyrosinate fluorescence emission in alpha- and beta-purothionins.

F. G. Prendergast, P. D. Hampton, B. Jones

Research output: Contribution to journalArticle

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Abstract

The CD, absorption, and fluorescence spectra and fluorescence lifetimes of three highly homologous, basic cytotoxic proteins isolated from wheat (alpha 1-, alpha 2-, and beta-purothionins) and a moderately homologous protein isolated from Crambe abyssinica (crambin) have been measured. The purothionins each contain a single tyrosine, while crambin has two tyrosine residues. At neutral pH in buffered solution or in water, beta-purothionin showed a single fluorescence emission peak maximal at 345 nm; alpha 1- and alpha 2-purothionins gave a double-humped emission (lambda max 308 and 345 nm), while crambin emitted only at 303 nm. Under acid pH conditions (less than pH 3) or when denatured in 6 M guanidine hydrochloride, the spectra of the alpha- and beta-purothionins showed predominantly the 303-nm emission (tau = 3.1 ns) while at pH greater than 10.0 only the 345-nm emission was evinced by all three proteins. Crambin showed typical tyrosine emission in the pH range 3-9 and weak tyrosinate fluorescence at pH greater than 10.5. From these features, and from the absorption and CD spectra, we infer that the 345-nm fluorescence emission of either alpha 1- or beta-purothionin is from tyrosinate moieties. The purothionin emission spectra appear to be generated by excited-state proton transfer rather than from tyrosinate species in the ground state.

Original languageEnglish (US)
Pages (from-to)6690-6697
Number of pages8
JournalBiochemistry
Volume23
Issue number26
StatePublished - Dec 18 1984

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Fluorescence
Crambe Plant
Tyrosine
Proteins
Proton transfer
purothionin
Guanidine
Excited states
Ground state
Triticum
Protons
Acids
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

Prendergast, F. G., Hampton, P. D., & Jones, B. (1984). Characteristics of tyrosinate fluorescence emission in alpha- and beta-purothionins. Biochemistry, 23(26), 6690-6697.

Characteristics of tyrosinate fluorescence emission in alpha- and beta-purothionins. / Prendergast, F. G.; Hampton, P. D.; Jones, B.

In: Biochemistry, Vol. 23, No. 26, 18.12.1984, p. 6690-6697.

Research output: Contribution to journalArticle

Prendergast, FG, Hampton, PD & Jones, B 1984, 'Characteristics of tyrosinate fluorescence emission in alpha- and beta-purothionins.', Biochemistry, vol. 23, no. 26, pp. 6690-6697.
Prendergast FG, Hampton PD, Jones B. Characteristics of tyrosinate fluorescence emission in alpha- and beta-purothionins. Biochemistry. 1984 Dec 18;23(26):6690-6697.
Prendergast, F. G. ; Hampton, P. D. ; Jones, B. / Characteristics of tyrosinate fluorescence emission in alpha- and beta-purothionins. In: Biochemistry. 1984 ; Vol. 23, No. 26. pp. 6690-6697.
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