Characterisation of hepatitis C virus genotype among blood donors at the regional blood transfusion centre of Ouagadougou, Burkina Faso

Moctar Tokèda Abdoul Zeba, Mahamoudou Sanou, Cyrille Bisseye, Alice Kiba, Bolni Nagalo, Florencia Wendkuuni Djigma, Tegwindé Rebecca Compaoré, Yacouba Koumpingnin Nebié, Kisito Kienou, Tani Sagna, Virginio Pietra, Rémy Moret, Jacques Simporé

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background. Hepatitis C virus (HCV) is responsible for about 900 deaths every year in Burkina Faso. In this country, serological screening for hepatitis B and C viruses is only carried out systematically among blood donors. The aim of this study was to determine the prevalence and genotypes of HCV among blood donors using reverse transcription polymerase chain reaction (PCR) and real-time PCR, respectively. Materials and methods. Serum samples were screened for antibodies to HCV using an enzymelinked immunosorbent assay (ARCHITECT-i1000SR- ABBOTT). All the reactive samples for HCV antibodies were re-tested using a second enzyme-linked immunosorbent assay (Bio-Rad, Marnes la Coquette, France) for confirmation. RNA was detected in all the reactive samples for antibodies to HCV. HCV RNA positive samples were genotyped using the HCV Real-TM Genotype kit (Sacace Biotechnologies, Italy). Results. Among 2,200 blood donors, the prevalences of antibodies to HCV and viral RNA were 4.4% (95% confidence interval=3.5-5.3) and 1.5% (95% confidence interval=1.0-2.0), respectively. Among HCV RNA carriers, genotyping showed that HCV genotypes 2 and 3 were the most prevalent as they were detected in 18 (56.3%) and 5 (15.6%) individuals, respectively. HCV genotypes 1a and 4 were the least frequent among the blood donors. HCV mixed genotypes 2/3 and 2/4 were also detected among the blood donors. Conclusion. The prevalence of HCV found in this study is lower than previously reported prevalences. Large-scale studies are needed to obtain a better picture of the molecular epidemiology of HCV in Burkina Faso.

Original languageEnglish (US)
JournalBlood Transfusion
Volume12
Issue numberSUPPL.1
DOIs
StatePublished - Feb 20 2014
Externally publishedYes

Fingerprint

Burkina Faso
Blood Donors
Blood Transfusion
Hepacivirus
Genotype
Hepatitis C Antibodies
RNA
Confidence Intervals
Immunosorbents
Molecular Epidemiology
Viral RNA
Biotechnology
Hepatitis B virus
Italy
Reverse Transcription
France
Real-Time Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay

Keywords

  • Blood donors.
  • Genotype
  • HCV
  • RNA

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy

Cite this

Characterisation of hepatitis C virus genotype among blood donors at the regional blood transfusion centre of Ouagadougou, Burkina Faso. / Zeba, Moctar Tokèda Abdoul; Sanou, Mahamoudou; Bisseye, Cyrille; Kiba, Alice; Nagalo, Bolni; Djigma, Florencia Wendkuuni; Compaoré, Tegwindé Rebecca; Nebié, Yacouba Koumpingnin; Kienou, Kisito; Sagna, Tani; Pietra, Virginio; Moret, Rémy; Simporé, Jacques.

In: Blood Transfusion, Vol. 12, No. SUPPL.1, 20.02.2014.

Research output: Contribution to journalArticle

Zeba, MTA, Sanou, M, Bisseye, C, Kiba, A, Nagalo, B, Djigma, FW, Compaoré, TR, Nebié, YK, Kienou, K, Sagna, T, Pietra, V, Moret, R & Simporé, J 2014, 'Characterisation of hepatitis C virus genotype among blood donors at the regional blood transfusion centre of Ouagadougou, Burkina Faso', Blood Transfusion, vol. 12, no. SUPPL.1. https://doi.org/10.2450/2013.0089-12
Zeba, Moctar Tokèda Abdoul ; Sanou, Mahamoudou ; Bisseye, Cyrille ; Kiba, Alice ; Nagalo, Bolni ; Djigma, Florencia Wendkuuni ; Compaoré, Tegwindé Rebecca ; Nebié, Yacouba Koumpingnin ; Kienou, Kisito ; Sagna, Tani ; Pietra, Virginio ; Moret, Rémy ; Simporé, Jacques. / Characterisation of hepatitis C virus genotype among blood donors at the regional blood transfusion centre of Ouagadougou, Burkina Faso. In: Blood Transfusion. 2014 ; Vol. 12, No. SUPPL.1.
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abstract = "Background. Hepatitis C virus (HCV) is responsible for about 900 deaths every year in Burkina Faso. In this country, serological screening for hepatitis B and C viruses is only carried out systematically among blood donors. The aim of this study was to determine the prevalence and genotypes of HCV among blood donors using reverse transcription polymerase chain reaction (PCR) and real-time PCR, respectively. Materials and methods. Serum samples were screened for antibodies to HCV using an enzymelinked immunosorbent assay (ARCHITECT-i1000SR- ABBOTT). All the reactive samples for HCV antibodies were re-tested using a second enzyme-linked immunosorbent assay (Bio-Rad, Marnes la Coquette, France) for confirmation. RNA was detected in all the reactive samples for antibodies to HCV. HCV RNA positive samples were genotyped using the HCV Real-TM Genotype kit (Sacace Biotechnologies, Italy). Results. Among 2,200 blood donors, the prevalences of antibodies to HCV and viral RNA were 4.4{\%} (95{\%} confidence interval=3.5-5.3) and 1.5{\%} (95{\%} confidence interval=1.0-2.0), respectively. Among HCV RNA carriers, genotyping showed that HCV genotypes 2 and 3 were the most prevalent as they were detected in 18 (56.3{\%}) and 5 (15.6{\%}) individuals, respectively. HCV genotypes 1a and 4 were the least frequent among the blood donors. HCV mixed genotypes 2/3 and 2/4 were also detected among the blood donors. Conclusion. The prevalence of HCV found in this study is lower than previously reported prevalences. Large-scale studies are needed to obtain a better picture of the molecular epidemiology of HCV in Burkina Faso.",
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T1 - Characterisation of hepatitis C virus genotype among blood donors at the regional blood transfusion centre of Ouagadougou, Burkina Faso

AU - Zeba, Moctar Tokèda Abdoul

AU - Sanou, Mahamoudou

AU - Bisseye, Cyrille

AU - Kiba, Alice

AU - Nagalo, Bolni

AU - Djigma, Florencia Wendkuuni

AU - Compaoré, Tegwindé Rebecca

AU - Nebié, Yacouba Koumpingnin

AU - Kienou, Kisito

AU - Sagna, Tani

AU - Pietra, Virginio

AU - Moret, Rémy

AU - Simporé, Jacques

PY - 2014/2/20

Y1 - 2014/2/20

N2 - Background. Hepatitis C virus (HCV) is responsible for about 900 deaths every year in Burkina Faso. In this country, serological screening for hepatitis B and C viruses is only carried out systematically among blood donors. The aim of this study was to determine the prevalence and genotypes of HCV among blood donors using reverse transcription polymerase chain reaction (PCR) and real-time PCR, respectively. Materials and methods. Serum samples were screened for antibodies to HCV using an enzymelinked immunosorbent assay (ARCHITECT-i1000SR- ABBOTT). All the reactive samples for HCV antibodies were re-tested using a second enzyme-linked immunosorbent assay (Bio-Rad, Marnes la Coquette, France) for confirmation. RNA was detected in all the reactive samples for antibodies to HCV. HCV RNA positive samples were genotyped using the HCV Real-TM Genotype kit (Sacace Biotechnologies, Italy). Results. Among 2,200 blood donors, the prevalences of antibodies to HCV and viral RNA were 4.4% (95% confidence interval=3.5-5.3) and 1.5% (95% confidence interval=1.0-2.0), respectively. Among HCV RNA carriers, genotyping showed that HCV genotypes 2 and 3 were the most prevalent as they were detected in 18 (56.3%) and 5 (15.6%) individuals, respectively. HCV genotypes 1a and 4 were the least frequent among the blood donors. HCV mixed genotypes 2/3 and 2/4 were also detected among the blood donors. Conclusion. The prevalence of HCV found in this study is lower than previously reported prevalences. Large-scale studies are needed to obtain a better picture of the molecular epidemiology of HCV in Burkina Faso.

AB - Background. Hepatitis C virus (HCV) is responsible for about 900 deaths every year in Burkina Faso. In this country, serological screening for hepatitis B and C viruses is only carried out systematically among blood donors. The aim of this study was to determine the prevalence and genotypes of HCV among blood donors using reverse transcription polymerase chain reaction (PCR) and real-time PCR, respectively. Materials and methods. Serum samples were screened for antibodies to HCV using an enzymelinked immunosorbent assay (ARCHITECT-i1000SR- ABBOTT). All the reactive samples for HCV antibodies were re-tested using a second enzyme-linked immunosorbent assay (Bio-Rad, Marnes la Coquette, France) for confirmation. RNA was detected in all the reactive samples for antibodies to HCV. HCV RNA positive samples were genotyped using the HCV Real-TM Genotype kit (Sacace Biotechnologies, Italy). Results. Among 2,200 blood donors, the prevalences of antibodies to HCV and viral RNA were 4.4% (95% confidence interval=3.5-5.3) and 1.5% (95% confidence interval=1.0-2.0), respectively. Among HCV RNA carriers, genotyping showed that HCV genotypes 2 and 3 were the most prevalent as they were detected in 18 (56.3%) and 5 (15.6%) individuals, respectively. HCV genotypes 1a and 4 were the least frequent among the blood donors. HCV mixed genotypes 2/3 and 2/4 were also detected among the blood donors. Conclusion. The prevalence of HCV found in this study is lower than previously reported prevalences. Large-scale studies are needed to obtain a better picture of the molecular epidemiology of HCV in Burkina Faso.

KW - Blood donors.

KW - Genotype

KW - HCV

KW - RNA

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