Changes in leucyl-tRNAs and aminoacyl tRNA synthetases in developing and aging soybean cotyledons

Vijayalakshmi Shridhar, Datta T.N. Pillay

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Leucine specific tRNA of soybean cotyledons was frationated into six peaks (1-6). The relative amounts of Leu-tRNA 5 and 6 are lower in developing cotyledons than in germinating cotyledons. Leu-tRNA synthetase from developing cotyledons is less active in aminoacylating Leu-tRNA 5 and 6 compared to enzyme from 5-day-old germinating cotyledons. Leu-tRNA synthetase from cotyledons of germinating seedlings and developing cotyledons can be fractionated into three peaks (1-3). Peak 1 in the developing cotyledon is about 36% less than peak 1 from 5-day-old germinating cotyledons. Peaks 2 and 3 from developing cotyledons are about 10 and 18% higher than from germinating cotyledons, respectively. Peak 1 from developing cotyledons acylates all six species of Leu-tRNA in contrast with peak 1 from germinating cotyledons, which essentially acylates only Leu-tRNA 5 and 6. The specificity of peaks 2 and 3 towards Leu-tRNA 1-4 is identical in both the organs.

Original languageEnglish (US)
Pages (from-to)1809-1812
Number of pages4
JournalPhytochemistry
Volume15
Issue number12
DOIs
StatePublished - 1976

Keywords

  • Glycine max
  • Leguminosae
  • hydroxylapatite column
  • leucyl transfer RNA synthetase
  • leucyl, transfer RNAs
  • reversed phase chromatography.
  • soybean

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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