Changes in interstitial cells of cajal with age in the human stomach and colon

P. J. Gomez-Pinilla, S. J. Gibbons, M. G. Sarr, M. L. Kendrick, K. Robert Shen, R. R. Cima, Eric Dozois, David Larson, Tamas Ordog, M. J. Pozo, Gianrico Farrugia

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Background: Aging produces inevitable changes in the function of most organs including the gastrointestinal tract. Together with enteric nerves and smooth muscle cells, interstitial cells of Cajal (ICC) play a key role in the control of gastrointestinal motility, yet little is known about the effect of aging on ICC. The aim of this study was to determine the effect of aging on ICC number and volume in the human stomach and colon.Methods: Gastric and colonic tissues from patients aged 25-70 and 36-92 years old, respectively, and with no co- existent motility disorders were immunolabeled with an anti- Kit antibody and ICC were counted in the circular muscle and myenteric regions. Network volumes were measured using 3D reconstructions of confocal stacks. The effects of aging were determined by testing for linear trends using regression analysis.Key Results: In both stomach and colon, the number of ICC bodies and volume significantly decreased with age at a rate of 13% per decade. ICC size was only affected in the myenteric plexus in the colon. The changes associated with age were not differentially affected by sex or colonic region.Conclusions & Inferences: The number and volume of ICC networks in the normal human stomach and colon decline with age. This decrease in ICC likely reduces the functional capacity of the gastrointestinal motor apparatus, may contribute to changes in gastrointestinal motility with aging and may influence intestinal responses to insults such as disease, operative interventions and medications in older patients. Tissue specimens must be carefully age- matched when studying ICC in disease.

Original languageEnglish (US)
Pages (from-to)36-44
Number of pages9
JournalNeurogastroenterology and Motility
Volume23
Issue number1
DOIs
StatePublished - Jan 2011

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Interstitial Cells of Cajal
Stomach
Colon
Cell Size
Gastrointestinal Motility
Myenteric Plexus
Smooth Muscle Myocytes
Gastrointestinal Tract
Anti-Idiotypic Antibodies
Cell Count
Regression Analysis

Keywords

  • Healthy aging
  • Motility
  • Myenteric plexus

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology

Cite this

Gomez-Pinilla, P. J., Gibbons, S. J., Sarr, M. G., Kendrick, M. L., Robert Shen, K., Cima, R. R., ... Farrugia, G. (2011). Changes in interstitial cells of cajal with age in the human stomach and colon. Neurogastroenterology and Motility, 23(1), 36-44. https://doi.org/10.1111/j.1365-2982.2010.01590.x

Changes in interstitial cells of cajal with age in the human stomach and colon. / Gomez-Pinilla, P. J.; Gibbons, S. J.; Sarr, M. G.; Kendrick, M. L.; Robert Shen, K.; Cima, R. R.; Dozois, Eric; Larson, David; Ordog, Tamas; Pozo, M. J.; Farrugia, Gianrico.

In: Neurogastroenterology and Motility, Vol. 23, No. 1, 01.2011, p. 36-44.

Research output: Contribution to journalArticle

Gomez-Pinilla PJ, Gibbons SJ, Sarr MG, Kendrick ML, Robert Shen K, Cima RR et al. Changes in interstitial cells of cajal with age in the human stomach and colon. Neurogastroenterology and Motility. 2011 Jan;23(1):36-44. https://doi.org/10.1111/j.1365-2982.2010.01590.x
Gomez-Pinilla, P. J. ; Gibbons, S. J. ; Sarr, M. G. ; Kendrick, M. L. ; Robert Shen, K. ; Cima, R. R. ; Dozois, Eric ; Larson, David ; Ordog, Tamas ; Pozo, M. J. ; Farrugia, Gianrico. / Changes in interstitial cells of cajal with age in the human stomach and colon. In: Neurogastroenterology and Motility. 2011 ; Vol. 23, No. 1. pp. 36-44.
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