Cervical dorsal rhizotomy (CDR) enlarges phrenic motoneurons in rats

W. Z. Zhan, P. Zhan, Y.s. Prakash, R. Kinkead, K. Smithson, G. S. Mitchell, Gary C Sieck

Research output: Contribution to journalArticle

Abstract

We hypothesized that CDR (C3-C5) would alter the morphology of phrenic motoneurons, reflecting spinal plasticity. Adult male rats were studied 28 days after CDR (N=6) or sham surgery (N=6). Phrenic motoneurons were labeled by intramuscular injection of cholera toxin B-fragment (CTB) three days before the rats were sacrificed. CTB labeled phrenic motoneurons were visualized using an immunohistochemical reaction with a Cy-3 conjugated secondary antibody. Longitudinal sections were imaged using a Bio Rad MRC500 confocal microscope. A series of optical slices (0.6 mm) through the phrenic motoneuron pool were digitized and transferred to a Sun workstation where optical slices were spatially reconstructed using ANALYZE software. Cell volumes and surface areas for ̃80 motoneurons were measured and corrected for the estimated Z-axis distortion, based on 3-D reconstruction of fluorescent microspheres of known diameter. CDR increased cell volumes and surface areas of the motoneurons in comparison to sham-operated rats. These structural adaptations suggest that the intrinsic excitability of phrenic motoneurons may decrease following CDR; however, this hypothesis remains to be tested directly.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Rhizotomy
Motor Neurons
motor neurons
Diaphragm
Rats
Cholera Toxin
rats
cholera toxin
Microspheres
Sun
Surgery
Plasticity
Cell Size
Microscopes
surface area
Antibodies
Intramuscular Injections
Solar System
intramuscular injection
microscopes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Zhan, W. Z., Zhan, P., Prakash, Y. S., Kinkead, R., Smithson, K., Mitchell, G. S., & Sieck, G. C. (1997). Cervical dorsal rhizotomy (CDR) enlarges phrenic motoneurons in rats. FASEB Journal, 11(3).

Cervical dorsal rhizotomy (CDR) enlarges phrenic motoneurons in rats. / Zhan, W. Z.; Zhan, P.; Prakash, Y.s.; Kinkead, R.; Smithson, K.; Mitchell, G. S.; Sieck, Gary C.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Zhan, WZ, Zhan, P, Prakash, YS, Kinkead, R, Smithson, K, Mitchell, GS & Sieck, GC 1997, 'Cervical dorsal rhizotomy (CDR) enlarges phrenic motoneurons in rats', FASEB Journal, vol. 11, no. 3.
Zhan WZ, Zhan P, Prakash YS, Kinkead R, Smithson K, Mitchell GS et al. Cervical dorsal rhizotomy (CDR) enlarges phrenic motoneurons in rats. FASEB Journal. 1997;11(3).
Zhan, W. Z. ; Zhan, P. ; Prakash, Y.s. ; Kinkead, R. ; Smithson, K. ; Mitchell, G. S. ; Sieck, Gary C. / Cervical dorsal rhizotomy (CDR) enlarges phrenic motoneurons in rats. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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