Cerebral metabolic profile, selective neuron loss, and survival of acute and chronic hyperglycemic rats following cardiac arrest and resuscitation

Joseph M. Hoxworth, Kui Xu, Yinong Zhou, W. David Lust, Joseph C. Lamanna

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Cortical metabolites and regional cerebral intracellular pH (pH(i)) were measured in normoglycemic (NM), acute hyperglycemic (AH), and chronic hyperglycemic (CH, 2 week duration, streptozotocin-induced) Wistar rat brains during cardiac arrest and resuscitation. During total ischemia in AH and CH rats (plasma glucose ~ 30 mM), cortical ATP, PCr, glucose, and glycogen all fell significantly as expected. Lactate levels increased dramatically in association with a concomitant intracellular acidosis. Although lactate reached higher concentrations in AH and CH than NM, pH(i) was significantly lower only in the AH group. With 5 min of reperfusion, all groups recovered to near baseline in all variables, though lactate remained elevated. In a separate aspect of the study, animals from each experimental group were allowed to recover for 4 days following resuscitation, with outcome being gauged by mortality rate and hippocampal CA1 neuron counts. NM survival rate was significantly better than AH and CH. In particular, no CH rats survived for 4 days despite rapid initial recovery. After 4 days, the AH group had suffered significantly greater CA1 neuron loss than the NM rats. In summary, our research identified differences in intra-ischemic acid-base status in the two hyperglycemic groups, suggesting that chronic hyperglycemia may alter the brain's buffering capacity. These observations may account for differences between acutely and chronically hyperglycemic subjects regarding outcome, and they suggest that factors other than hydrogen ion production during ischemia are responsible for modulating outcome.

Original languageEnglish (US)
Pages (from-to)467-479
Number of pages13
JournalBrain Research
Volume821
Issue number2
DOIs
StatePublished - Mar 13 1999
Externally publishedYes

Fingerprint

Metabolome
Heart Arrest
Resuscitation
Lactic Acid
Neurons
Ischemia
Glucose
Brain
Streptozocin
Acidosis
Glycogen
Hyperglycemia
Reperfusion
Protons
Wistar Rats
Survival Rate
Adenosine Triphosphate
Acids
Mortality
Research

Keywords

  • Global complete cerebral ischemia
  • High-energy phosphate
  • Intracellular pH
  • Lactic acidosis
  • Selective neuronal vulnerability
  • Streptozotocin-induced diabetes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cerebral metabolic profile, selective neuron loss, and survival of acute and chronic hyperglycemic rats following cardiac arrest and resuscitation. / Hoxworth, Joseph M.; Xu, Kui; Zhou, Yinong; Lust, W. David; Lamanna, Joseph C.

In: Brain Research, Vol. 821, No. 2, 13.03.1999, p. 467-479.

Research output: Contribution to journalArticle

Hoxworth, Joseph M. ; Xu, Kui ; Zhou, Yinong ; Lust, W. David ; Lamanna, Joseph C. / Cerebral metabolic profile, selective neuron loss, and survival of acute and chronic hyperglycemic rats following cardiac arrest and resuscitation. In: Brain Research. 1999 ; Vol. 821, No. 2. pp. 467-479.
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