Centrin1 is required for organelle segregation and cytokinesis in Trypanosoma brucei

Angamuthu Selvapandiyan, Praveen Kumar, James C. Morris, Jeffrey L Salisbury, Ching C. Wang, Hira L. Nakhasi

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Centrin is a calcium-binding centrosome/basal body-associated protein involved in duplication and segregation of these organelles in eukaryotes. We had shown that disruption of one of the centrin genes (centrin1) in Leishmania amastigotes resulted in failure of both basal body duplication and cytokinesis. Here, we undertook to define the role of centrin1 (TbCen1) in the duplication and segregation of basal body and its associated organelles kinetoplast and Golgi, as well as its role in cytokinesis of the procyclic form of Trypanosoma brucei by depleting its protein using RNA inhibition methodology. TbCen1-depleted cells showed significant reduction in growth compared with control cells. Morphological analysis of these cells showed they were large and pleomorphic with multiple detached flagella. Both immunofluorescence assays using organelle-specific antibodies and electron microscopic analysis showed that TbCen1-deficient cells contained multiple basal bodies, kinetoplasts, Golgi, and nuclei. These multiple organelles were, however, closely clustered together, indicating duplication without segregation in the absence of centrin. This failure in organelle segregation may be the likely cause of inhibition of cytokinesis, suggesting for the first time a new and unique role for centrin in the segregation of organelles without affecting their multiplication in the procyclic form of T. brucei.

Original languageEnglish (US)
Pages (from-to)3290-3301
Number of pages12
JournalMolecular Biology of the Cell
Volume18
Issue number9
DOIs
StatePublished - Sep 2007

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Trypanosoma brucei brucei
Cytokinesis
Organelles
Basal Bodies
Sulfamethoxazole Drug Combination Trimethoprim
Centrosome
Flagella
Leishmania
Eukaryota
Fluorescent Antibody Technique
Proteins
RNA
Electrons
Calcium
Antibodies
Growth
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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Centrin1 is required for organelle segregation and cytokinesis in Trypanosoma brucei. / Selvapandiyan, Angamuthu; Kumar, Praveen; Morris, James C.; Salisbury, Jeffrey L; Wang, Ching C.; Nakhasi, Hira L.

In: Molecular Biology of the Cell, Vol. 18, No. 9, 09.2007, p. 3290-3301.

Research output: Contribution to journalArticle

Selvapandiyan, Angamuthu ; Kumar, Praveen ; Morris, James C. ; Salisbury, Jeffrey L ; Wang, Ching C. ; Nakhasi, Hira L. / Centrin1 is required for organelle segregation and cytokinesis in Trypanosoma brucei. In: Molecular Biology of the Cell. 2007 ; Vol. 18, No. 9. pp. 3290-3301.
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