Centrin-2 is required for centriole duplication in mammalian cells

Jeffrey L Salisbury, Kelly M. Suino, Robert Busby, Margaret Springett

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Background: Centrosomes are the favored microtubule-organizing framework of eukaryotic cells. Centrosomes contain a pair of centrioles that normally duplicate once during the cell cycle to give rise to two mitotic spindle poles, each containing one old and one new centriole. However, aside from their role as an anchor point for pericentriolar material and as basal bodies of flagella and cilia, the functional attributes of centrioles remain enigmatic. Results: Here, using RNA interference, we demonstrate that "knockdown" of centrin-2, a protein of centrioles, results in failure of centriole duplication during the cell cycle in HeLa cells. Following inhibition of centrin-2 synthesis, the preexisting pair of centrioles separate, and functional bipolar spindles form with only one centriole at each spindle pole. Centriole dilution results from the ensuing cell division, and daughter cells are "born" with only a single centriole. Remarkably, these unicentriolar daughter cells may complete a second and even third bipolar mitosis in which spindle microtubules converge onto unusually broad spindle poles and in which cell division results in daughter cells containing either one or no centrioles at all. Cells thus denuded of the mature or both centrioles fail to undergo cytokinesis in subsequent cell cycles, give rise to multinucleate products, and finally die. Conclusions: These results demonstrate a requirement for centrin in centriole duplication and demonstrate that centrioles play a role in organizing spindle pole morphology and in the completion of cytokinesis.

Original languageEnglish (US)
Pages (from-to)1287-1292
Number of pages6
JournalCurrent Biology
Volume12
Issue number15
DOIs
StatePublished - Aug 6 2002

Fingerprint

Centrioles
centrioles
Sulfamethoxazole Drug Combination Trimethoprim
Cells
Poles
Spindle Poles
cells
Anchors
centrosomes
cell cycle
Centrosome
Cell Cycle
Dilution
Cytokinesis
cytokinesis
Microtubules
Cell Division
RNA
microtubules
cell division

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Centrin-2 is required for centriole duplication in mammalian cells. / Salisbury, Jeffrey L; Suino, Kelly M.; Busby, Robert; Springett, Margaret.

In: Current Biology, Vol. 12, No. 15, 06.08.2002, p. 1287-1292.

Research output: Contribution to journalArticle

Salisbury, Jeffrey L ; Suino, Kelly M. ; Busby, Robert ; Springett, Margaret. / Centrin-2 is required for centriole duplication in mammalian cells. In: Current Biology. 2002 ; Vol. 12, No. 15. pp. 1287-1292.
@article{8b669a2e6a8c499ea57cb10511d4a7c8,
title = "Centrin-2 is required for centriole duplication in mammalian cells",
abstract = "Background: Centrosomes are the favored microtubule-organizing framework of eukaryotic cells. Centrosomes contain a pair of centrioles that normally duplicate once during the cell cycle to give rise to two mitotic spindle poles, each containing one old and one new centriole. However, aside from their role as an anchor point for pericentriolar material and as basal bodies of flagella and cilia, the functional attributes of centrioles remain enigmatic. Results: Here, using RNA interference, we demonstrate that {"}knockdown{"} of centrin-2, a protein of centrioles, results in failure of centriole duplication during the cell cycle in HeLa cells. Following inhibition of centrin-2 synthesis, the preexisting pair of centrioles separate, and functional bipolar spindles form with only one centriole at each spindle pole. Centriole dilution results from the ensuing cell division, and daughter cells are {"}born{"} with only a single centriole. Remarkably, these unicentriolar daughter cells may complete a second and even third bipolar mitosis in which spindle microtubules converge onto unusually broad spindle poles and in which cell division results in daughter cells containing either one or no centrioles at all. Cells thus denuded of the mature or both centrioles fail to undergo cytokinesis in subsequent cell cycles, give rise to multinucleate products, and finally die. Conclusions: These results demonstrate a requirement for centrin in centriole duplication and demonstrate that centrioles play a role in organizing spindle pole morphology and in the completion of cytokinesis.",
author = "Salisbury, {Jeffrey L} and Suino, {Kelly M.} and Robert Busby and Margaret Springett",
year = "2002",
month = "8",
day = "6",
doi = "10.1016/S0960-9822(02)01019-9",
language = "English (US)",
volume = "12",
pages = "1287--1292",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "15",

}

TY - JOUR

T1 - Centrin-2 is required for centriole duplication in mammalian cells

AU - Salisbury, Jeffrey L

AU - Suino, Kelly M.

AU - Busby, Robert

AU - Springett, Margaret

PY - 2002/8/6

Y1 - 2002/8/6

N2 - Background: Centrosomes are the favored microtubule-organizing framework of eukaryotic cells. Centrosomes contain a pair of centrioles that normally duplicate once during the cell cycle to give rise to two mitotic spindle poles, each containing one old and one new centriole. However, aside from their role as an anchor point for pericentriolar material and as basal bodies of flagella and cilia, the functional attributes of centrioles remain enigmatic. Results: Here, using RNA interference, we demonstrate that "knockdown" of centrin-2, a protein of centrioles, results in failure of centriole duplication during the cell cycle in HeLa cells. Following inhibition of centrin-2 synthesis, the preexisting pair of centrioles separate, and functional bipolar spindles form with only one centriole at each spindle pole. Centriole dilution results from the ensuing cell division, and daughter cells are "born" with only a single centriole. Remarkably, these unicentriolar daughter cells may complete a second and even third bipolar mitosis in which spindle microtubules converge onto unusually broad spindle poles and in which cell division results in daughter cells containing either one or no centrioles at all. Cells thus denuded of the mature or both centrioles fail to undergo cytokinesis in subsequent cell cycles, give rise to multinucleate products, and finally die. Conclusions: These results demonstrate a requirement for centrin in centriole duplication and demonstrate that centrioles play a role in organizing spindle pole morphology and in the completion of cytokinesis.

AB - Background: Centrosomes are the favored microtubule-organizing framework of eukaryotic cells. Centrosomes contain a pair of centrioles that normally duplicate once during the cell cycle to give rise to two mitotic spindle poles, each containing one old and one new centriole. However, aside from their role as an anchor point for pericentriolar material and as basal bodies of flagella and cilia, the functional attributes of centrioles remain enigmatic. Results: Here, using RNA interference, we demonstrate that "knockdown" of centrin-2, a protein of centrioles, results in failure of centriole duplication during the cell cycle in HeLa cells. Following inhibition of centrin-2 synthesis, the preexisting pair of centrioles separate, and functional bipolar spindles form with only one centriole at each spindle pole. Centriole dilution results from the ensuing cell division, and daughter cells are "born" with only a single centriole. Remarkably, these unicentriolar daughter cells may complete a second and even third bipolar mitosis in which spindle microtubules converge onto unusually broad spindle poles and in which cell division results in daughter cells containing either one or no centrioles at all. Cells thus denuded of the mature or both centrioles fail to undergo cytokinesis in subsequent cell cycles, give rise to multinucleate products, and finally die. Conclusions: These results demonstrate a requirement for centrin in centriole duplication and demonstrate that centrioles play a role in organizing spindle pole morphology and in the completion of cytokinesis.

UR - http://www.scopus.com/inward/record.url?scp=0037031146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037031146&partnerID=8YFLogxK

U2 - 10.1016/S0960-9822(02)01019-9

DO - 10.1016/S0960-9822(02)01019-9

M3 - Article

C2 - 12176356

AN - SCOPUS:0037031146

VL - 12

SP - 1287

EP - 1292

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 15

ER -