Cellular stress inhibits vascular smooth muscle relaxation

Louis Knoepp, Arthur Beall, David A Woodrum, J. Sheppard Mondy, Ellen Shaver, Mary Dickinson, Colleen M. Brophy

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Purpose: Cellular stress has been shown to induce a group of proteins called heat shock proteins (HSPs). Recent evidence suggests that a group of small HSPs may modulate vascular smooth muscle contraction (HSP27) and/or relaxation (HSP20). In this investigation, we hypothesized that cellular stress would alter contraction and/or relaxation of intact vascular smooth muscles and would lead to changes in the induction and/or phosphorylation of the small HSPs. Methods: Bovine carotid arteries were obtained from an abattoir, and physiologic contractile responses were determined in a muscle bath. Phosphorylation state-specific antibodies were produced and characterized against HSP27. Phosphorylation events were determined with phosphorylation state-specific antibodies or whole-cell phosphorylation and two-dimensional gel electrophoresis. Results: Cellular stress induced by arsenite or heat shock did not alter basal tone or the magnitude of contractions induced by serotonin or high extracellular potassium chloride. However, cellular stress led to inhibition of forskolin and sodium nitroprusside-induced vasorelaxation. This impaired vasorelaxation was associated with increases in the phosphorylation of HSP27 and decreases in forskolin-induced phosphorylation of HSP20. Conclusion: Cellular stress, which leads to increases in the phosphorylation of HSP27, inhibits cyclic nucleotide-dependent vascular relaxation and cyclic nucleotide-dependent increases in the phosphorylation of HSP20.

Original languageEnglish (US)
Pages (from-to)343-353
Number of pages11
JournalJournal of Vascular Surgery
Volume31
Issue number2
StatePublished - 2000
Externally publishedYes

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Muscle Relaxation
Vascular Smooth Muscle
Phosphorylation
Phospho-Specific Antibodies
Small Heat-Shock Proteins
Cyclic Nucleotides
Colforsin
Vasodilation
Abattoirs
Potassium Chloride
Electrophoresis, Gel, Two-Dimensional
Nitroprusside
Muscle Contraction
Heat-Shock Proteins
Baths
Carotid Arteries
Blood Vessels
Shock
Serotonin
Hot Temperature

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Knoepp, L., Beall, A., Woodrum, D. A., Mondy, J. S., Shaver, E., Dickinson, M., & Brophy, C. M. (2000). Cellular stress inhibits vascular smooth muscle relaxation. Journal of Vascular Surgery, 31(2), 343-353.

Cellular stress inhibits vascular smooth muscle relaxation. / Knoepp, Louis; Beall, Arthur; Woodrum, David A; Mondy, J. Sheppard; Shaver, Ellen; Dickinson, Mary; Brophy, Colleen M.

In: Journal of Vascular Surgery, Vol. 31, No. 2, 2000, p. 343-353.

Research output: Contribution to journalArticle

Knoepp, L, Beall, A, Woodrum, DA, Mondy, JS, Shaver, E, Dickinson, M & Brophy, CM 2000, 'Cellular stress inhibits vascular smooth muscle relaxation', Journal of Vascular Surgery, vol. 31, no. 2, pp. 343-353.
Knoepp L, Beall A, Woodrum DA, Mondy JS, Shaver E, Dickinson M et al. Cellular stress inhibits vascular smooth muscle relaxation. Journal of Vascular Surgery. 2000;31(2):343-353.
Knoepp, Louis ; Beall, Arthur ; Woodrum, David A ; Mondy, J. Sheppard ; Shaver, Ellen ; Dickinson, Mary ; Brophy, Colleen M. / Cellular stress inhibits vascular smooth muscle relaxation. In: Journal of Vascular Surgery. 2000 ; Vol. 31, No. 2. pp. 343-353.
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