Cellular response of ovarian carcinoma cells to antibody-photosensitizer-mediated injury

T. Hasan, M. E. Sherwood, T. Anderson, M. Bamberg, T. J. Flotte, V. R. Zurawski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

An anti-ovarian carcinoma antibody OC125 was conjugated to a derivative of the photosensitizer (PS) chlorin e6 via polyglutamic acid. Target cells from a human ovarian cancer cell line were treated with this conjugate and laser irradiation at 656 nm (absorption maximum of PS) and fixed 24 h later for electron microscopy. Electron micrographs showed a high degree of vacoulization, generalized cell necrosis, and extrusion of organelles. No specific damage to the plasma membrane was noted. Untreated control cells, or cells treated with conjugate or light alone exhibited no injury. These data suggest that even though the antibody recognizes a cell surface antigen, the conjugate is internalized under the conditions of the experiment.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages136-152
Number of pages17
ISBN (Print)0819402443, 9780819402448
DOIs
StatePublished - 1990
Externally publishedYes
EventProceedings of Photodynamic Therapy: Mechanisms II - Los Angeles, CA, USA
Duration: Jan 16 1990Jan 17 1990

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1203
ISSN (Print)0277-786X

Other

OtherProceedings of Photodynamic Therapy: Mechanisms II
CityLos Angeles, CA, USA
Period1/16/901/17/90

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Hasan, T., Sherwood, M. E., Anderson, T., Bamberg, M., Flotte, T. J., & Zurawski, V. R. (1990). Cellular response of ovarian carcinoma cells to antibody-photosensitizer-mediated injury. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 136-152). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1203). Publ by Int Soc for Optical Engineering. https://doi.org/10.1117/12.17657