Cellular receptors for matrix proteins in normal human kidney and human mesangial cells

Fernando G Cosio, Daniel D. Sedmak, N. Stanley Nahman

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

In the present study we evaluated the distribution of cell adhesion molecules, referred as very late antigens (VLA), in the normal human kidney and in mesangial cells in culture (MC). In addition, we assessed the functional properties of VLA proteins on MC. Normal human kidney and MC were stained by immunoperoxidase with mouse monoclonal antibodies to VLA proteins. We demonstrated that VLA-3, a protein that binds FN, laminin and collagen, is the predominant VLA protein in the human glomerulus and on MC. VLA-3 is located in the mesangium and on the glomerular visceral epithelial cell and endothelial cell surfaces in contact with the glomerular basement membrane. VLA-1 was demonstrated in the glomerular mesangium and VLA-5, an FN specific receptor, was present in the mesangium, on glomerular endothelial cells and on MC. VLA-2 and VLA-4 were not present in the normal glomerulus nor on MC. In functional studies we evaluated the binding of MC to FN coated surfaces and the binding and phagocytosis of FN coated fluorescent beads by MC. We showed that MC bind to FN coated surfaces and that the binding is inhibited by anti-FN antibodies, EDTA and peptides containing the amino acid sequence Arg-Gly-Asp (RGD). In addition, anti-VLA-5 but not anti-VLA-3 antibodies inhibited significantly the binding of MC to FN. MC demonstrated binding and phagocytosis of FN coated beads and, purified FN inhibited both phenomena. By affinity chromatography and immunoprecipitation we demonstrated that MC FN binding proteins and MC VLA proteins are composed of two distinct protein chains that have Mr characteristics similar to those of normal human fibroblasts VLA proteins. In conclusion, the glomerular distribution of VLA-3 suggests that this protein is primarily involved on the adhesion of glomerular cells to basement membranes and matrix. MC FN receptors (VLA-5) mediate the binding of MC to FN and could mediate the phagocytosis of FN coated antigen or immune complexes by mesangial cells.

Original languageEnglish (US)
Pages (from-to)886-895
Number of pages10
JournalKidney International
Volume38
Issue number5
StatePublished - Nov 1990
Externally publishedYes

Fingerprint

Mesangial Cells
Antigens
Proteins
Glomerular Mesangium
Phagocytosis
Very Late Antigen Receptors
Integrin alpha2beta1
Endothelial Cells
Integrin alpha4beta1
Podocytes
Glomerular Basement Membrane
Cell Adhesion Molecules
Laminin
Antigen-Antibody Complex
Affinity Chromatography
Immunoprecipitation
Basement Membrane
Cell Adhesion
Edetic Acid
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Nephrology

Cite this

Cosio, F. G., Sedmak, D. D., & Nahman, N. S. (1990). Cellular receptors for matrix proteins in normal human kidney and human mesangial cells. Kidney International, 38(5), 886-895.

Cellular receptors for matrix proteins in normal human kidney and human mesangial cells. / Cosio, Fernando G; Sedmak, Daniel D.; Nahman, N. Stanley.

In: Kidney International, Vol. 38, No. 5, 11.1990, p. 886-895.

Research output: Contribution to journalArticle

Cosio, FG, Sedmak, DD & Nahman, NS 1990, 'Cellular receptors for matrix proteins in normal human kidney and human mesangial cells', Kidney International, vol. 38, no. 5, pp. 886-895.
Cosio, Fernando G ; Sedmak, Daniel D. ; Nahman, N. Stanley. / Cellular receptors for matrix proteins in normal human kidney and human mesangial cells. In: Kidney International. 1990 ; Vol. 38, No. 5. pp. 886-895.
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