Cellular immunologic studies of a patient with monocytic leukemia

Samuel K. Ackerman, Thomas F. Bumol, Neil Elliot Kay, Steven D. Douglas

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cellular immunologic studies were performed on the leukemic cells of a 59 year old white man with monocytic leukemia. Morphologically, most circulating leukocytes were monocytes. They demonstrated Fc and C3 (third component of complement) receptors, phagocytized latex particles, showed In vitro cytoplasmic spreading and lysed antibody-coated chicken erythrocytes. Phagocytosis of, as well as rosetting with, C3-coated erythrocytes and very rapid cytoplasmic spreading suggested in vivo monocyte "activation." The cells were easily maintained in primary culture for up to 13 weeks, with acquisition of typical macrophage morphology. In addition, nearly all cells demonstrated surface immunoglobulin (Slg) which was (1) trypsin-sensitive and did not regenerate in culture, (2) could be restored after trypsinization by incubation of cells In autologous or normal serum and (3) persisted in culture on 75 per cent of cells for at least seven days. Fluoresceinated monospecific antiserums showed an immunoglobulin G (IgG), kappa pattern on one occasion and an IgG, kappa, lambda pattern on another. Eluates of the cells prepared on three occasions showed only IgG and kappa reactivity using numerous antiserums. We concluded that most Slg was probably Fc-receptor bound with unusually high affinity and with greater representation of IgG (kappa) than IgG (lambda). These studies suggest that apparent "monoclonal" Slg does not necessarily indicate a truly clonal proliferation of B cell origin.

Original languageEnglish (US)
Pages (from-to)1061-1068
Number of pages8
JournalThe American journal of medicine
Volume64
Issue number6
DOIs
StatePublished - 1978
Externally publishedYes

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Leukemia
Immunoglobulin G
Immune Sera
Monocytes
Erythrocytes
Complement Receptors
B-Cell Antigen Receptors
Fc Receptors
Microspheres
Phagocytosis
Trypsin
Chickens
Leukocytes
B-Lymphocytes
Macrophages
Antibodies
Serum

ASJC Scopus subject areas

  • Nursing(all)

Cite this

Cellular immunologic studies of a patient with monocytic leukemia. / Ackerman, Samuel K.; Bumol, Thomas F.; Kay, Neil Elliot; Douglas, Steven D.

In: The American journal of medicine, Vol. 64, No. 6, 1978, p. 1061-1068.

Research output: Contribution to journalArticle

Ackerman, Samuel K. ; Bumol, Thomas F. ; Kay, Neil Elliot ; Douglas, Steven D. / Cellular immunologic studies of a patient with monocytic leukemia. In: The American journal of medicine. 1978 ; Vol. 64, No. 6. pp. 1061-1068.
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