Cellular identification and quantification of senescence-associated β-galactosidase activity in vivo

Bennett G. Childs, Tyler J. Bussian, Darren J Baker

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Senescence-associated β-galactosidase (hereafter SA-β-gal) staining has now been employed for more than 20 years to identify the presence of senescent cells (Dimri et al., Proc Natl Acad Sci U S A 92:9363–9367, 1995). These cells, characterized by a permanent cell-cycle arrest (Hayflick and Moorhead, Exp Cell Res 25:585–621, 1961) and the production of a distinct secretory phenotype of cytokines, chemokines, and proteases (Coppe et al., PLoS Biol 6:2853–2868, 2008), have received much attention in recent years for their impacts on diverse biological processes. Here we describe a method to identify and quantify the specific cells that become senescent in vivo using transmission electron microscopy after SA-β-gal staining that can be used in countless scenarios.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages31-38
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1896
ISSN (Print)1064-3745

Fingerprint

Galactosidases
Staining and Labeling
Biological Phenomena
Cell Cycle Checkpoints
Transmission Electron Microscopy
Chemokines
Peptide Hydrolases
Cytokines
Phenotype

Keywords

  • Cellular senescence
  • Senescence-associated β-galactosidase
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Childs, B. G., Bussian, T. J., & Baker, D. J. (2019). Cellular identification and quantification of senescence-associated β-galactosidase activity in vivo. In Methods in Molecular Biology (pp. 31-38). (Methods in Molecular Biology; Vol. 1896). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8931-7_4

Cellular identification and quantification of senescence-associated β-galactosidase activity in vivo. / Childs, Bennett G.; Bussian, Tyler J.; Baker, Darren J.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 31-38 (Methods in Molecular Biology; Vol. 1896).

Research output: Chapter in Book/Report/Conference proceedingChapter

Childs, BG, Bussian, TJ & Baker, DJ 2019, Cellular identification and quantification of senescence-associated β-galactosidase activity in vivo. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1896, Humana Press Inc., pp. 31-38. https://doi.org/10.1007/978-1-4939-8931-7_4
Childs BG, Bussian TJ, Baker DJ. Cellular identification and quantification of senescence-associated β-galactosidase activity in vivo. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 31-38. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8931-7_4
Childs, Bennett G. ; Bussian, Tyler J. ; Baker, Darren J. / Cellular identification and quantification of senescence-associated β-galactosidase activity in vivo. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 31-38 (Methods in Molecular Biology).
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