@inbook{94b1b7221aa64486a2ded50353eebd74,
title = "Cellular identification and quantification of senescence-associated β-galactosidase activity in vivo",
abstract = "Senescence-associated β-galactosidase (hereafter SA-β-gal) staining has now been employed for more than 20 years to identify the presence of senescent cells (Dimri et al., Proc Natl Acad Sci U S A 92:9363–9367, 1995). These cells, characterized by a permanent cell-cycle arrest (Hayflick and Moorhead, Exp Cell Res 25:585–621, 1961) and the production of a distinct secretory phenotype of cytokines, chemokines, and proteases (Coppe et al., PLoS Biol 6:2853–2868, 2008), have received much attention in recent years for their impacts on diverse biological processes. Here we describe a method to identify and quantify the specific cells that become senescent in vivo using transmission electron microscopy after SA-β-gal staining that can be used in countless scenarios.",
keywords = "Cellular senescence, Senescence-associated β-galactosidase, Transmission electron microscopy",
author = "Childs, {Bennett G.} and Bussian, {Tyler J.} and Baker, {Darren J.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-8931-7_4",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "31--38",
booktitle = "Methods in Molecular Biology",
}