Cellular identification and quantification of senescence-associated β-galactosidase activity in vivo

Bennett G. Childs, Tyler J. Bussian, Darren J. Baker

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Senescence-associated β-galactosidase (hereafter SA-β-gal) staining has now been employed for more than 20 years to identify the presence of senescent cells (Dimri et al., Proc Natl Acad Sci U S A 92:9363–9367, 1995). These cells, characterized by a permanent cell-cycle arrest (Hayflick and Moorhead, Exp Cell Res 25:585–621, 1961) and the production of a distinct secretory phenotype of cytokines, chemokines, and proteases (Coppe et al., PLoS Biol 6:2853–2868, 2008), have received much attention in recent years for their impacts on diverse biological processes. Here we describe a method to identify and quantify the specific cells that become senescent in vivo using transmission electron microscopy after SA-β-gal staining that can be used in countless scenarios.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages31-38
Number of pages8
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1896
ISSN (Print)1064-3745

Keywords

  • Cellular senescence
  • Senescence-associated β-galactosidase
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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