Cellular distribution of secretin receptor expression in rat pancreas

Charles D. Ulrich, Paul Wood, Elizabeth M. Hadac, Elizabeth Kopras, David C. Whitcomb, Laurence J Miller

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Secretin is an important regulator of pancreatic function, but the molecular basis of its actions is not well understood. We have, therefore, used in situ autoradiography, photoaffinity labeling, and RNase protection assays with healthy rat pancreas, dispersed acinar cells, and pancreas depleted of acinar cells to explore the cellular distribution and molecular identity of high-affinity secretin receptors in this complex organ. The autoradiographic examination of 125I-labeled [Tyr10]rat secretin-27 binding to normal pancreas demonstrated saturable and specific high-affinity binding sites on both acinar and duct cells, with a uniform lobular distribution, but with no binding above background over islets or vascular structures. Photoaffinity labeling demonstrated that the ductular binding site in acinar cell-depleted copper-deficient rat pancreas represented the same glycoprotein with a molecular weight of 50,000-62,000 that was present on acinar cells. RNase protection assays confirmed the molecular identity of the secretin receptors expressed on these distinct cells. The apparent absence or extreme low density of similar secretin receptors on islets and pancreatic vascular structures suggests that the pharmacological effects of secretin on those cells may either be indirect or mediated by another secretin family receptor that recognizes this hormone with lower affinity.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume275
Issue number6 38-6
StatePublished - 1998

Fingerprint

Acinar Cells
Pancreas
Secretin
Ribonucleases
Blood Vessels
Binding Sites
Autoradiography
Islets of Langerhans
Copper
Glycoproteins
Molecular Weight
secretin receptor
Hormones
Pharmacology

Keywords

  • Copper- deficient rat
  • Hormone receptor
  • Peptide hormone
  • Receptor autoradiography

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Cellular distribution of secretin receptor expression in rat pancreas. / Ulrich, Charles D.; Wood, Paul; Hadac, Elizabeth M.; Kopras, Elizabeth; Whitcomb, David C.; Miller, Laurence J.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 275, No. 6 38-6, 1998.

Research output: Contribution to journalArticle

Ulrich, Charles D. ; Wood, Paul ; Hadac, Elizabeth M. ; Kopras, Elizabeth ; Whitcomb, David C. ; Miller, Laurence J. / Cellular distribution of secretin receptor expression in rat pancreas. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1998 ; Vol. 275, No. 6 38-6.
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