TY - JOUR
T1 - Cellular and subcellular localization of the neuron-specific plasma membrane calcium ATPase PMCA1a in the rat brain
AU - Kenyon, Katharine A.
AU - Bushong, Eric A.
AU - Mauer, Amy S.
AU - Strehler, Emanuel E.
AU - Weinberg, Richard J.
AU - Burette, Alain C.
PY - 2010/8/15
Y1 - 2010/8/15
N2 - Regulation of intracellular calcium is crucial both for proper neuronal function and survival. By coupling ATP hydrolysis with Ca2+ extrusion from the cell, the plasma membrane calcium-dependent ATPases (PMCAs) play an essential role in controlling intracellular calcium levels in neurons. In contrast to PMCA2 and PMCA3, which are expressed in significant levels only in the brain and a few other tissues, PMCA1 is ubiquitously distributed, and is thus widely believed to play a "housekeeping" function in mammalian cells. Whereas the PMCA1b splice variant is predominant in most tissues, an alternative variant, PMCA1a, is the major form of PMCA1 in the adult brain. Here, we use immunohistochemistry to analyze the cellular and subcellular distribution of PMCA1a in the brain. We show that PMCA1a is not ubiquitously expressed, but rather is confined to neurons, where it concentrates in the plasma membrane of somata, dendrites, and spines. Thus, rather than serving a general housekeeping function, our data suggest that PMCA1a is a calcium pump specialized for neurons, where it may contribute to the modulation of somatic and dendritic Ca2+ transients.
AB - Regulation of intracellular calcium is crucial both for proper neuronal function and survival. By coupling ATP hydrolysis with Ca2+ extrusion from the cell, the plasma membrane calcium-dependent ATPases (PMCAs) play an essential role in controlling intracellular calcium levels in neurons. In contrast to PMCA2 and PMCA3, which are expressed in significant levels only in the brain and a few other tissues, PMCA1 is ubiquitously distributed, and is thus widely believed to play a "housekeeping" function in mammalian cells. Whereas the PMCA1b splice variant is predominant in most tissues, an alternative variant, PMCA1a, is the major form of PMCA1 in the adult brain. Here, we use immunohistochemistry to analyze the cellular and subcellular distribution of PMCA1a in the brain. We show that PMCA1a is not ubiquitously expressed, but rather is confined to neurons, where it concentrates in the plasma membrane of somata, dendrites, and spines. Thus, rather than serving a general housekeeping function, our data suggest that PMCA1a is a calcium pump specialized for neurons, where it may contribute to the modulation of somatic and dendritic Ca2+ transients.
KW - Cell Centered Database
KW - ImageSurfer
KW - calcium extrusion
KW - calcium pump
KW - immunohistochemistry
UR - http://www.scopus.com/inward/record.url?scp=77954605150&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954605150&partnerID=8YFLogxK
U2 - 10.1002/cne.22409
DO - 10.1002/cne.22409
M3 - Article
C2 - 20575074
AN - SCOPUS:77954605150
SN - 0021-9967
VL - 518
SP - 3169
EP - 3183
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
IS - 16
ER -