Cell migration and invasion assays

Aline Valster; Nhan L. Tran; Mitsutoshi Nakada; Michael E. Berens; Amanda Y. Chan; Marc Symons

doi:10.1016/j.ymeth.2005.08.001

Cell migration and invasion assays

Aline Valster, Nhan L. Tran, Mitsutoshi Nakada, Michael E. Berens, Amanda Y. Chan, Marc Symons

Research output: Contribution to journal › Article › peer-review

236 Scopus citations

Abstract

The processes of cell migration and invasion are integral to embryonic development and the functioning of adult organisms. Deregulation of these processes contributes to numerous diseases. Ras GTPases and in particular members of the Rho subfamily of GTPases play critical roles in cell migration and invasion. Here, we provide a collection of protocols to assay these functions. We describe two cell migration assays. The monolayer wound healing assay is very easy to implement, whereas the microliter-scale migration assay allows examination of cell behavior on defined extracellular matrices. We also describe two methods that allow the quantification of tumor cell invasion, a versatile transwell Matrigel invasion assay and an organotypic assay that examines the invasion of glioma cells through a rat brain slice.

Original language	English (US)
Pages (from-to)	208-215
Number of pages	8
Journal	Methods
Volume	37
Issue number	2
DOIs	https://doi.org/10.1016/j.ymeth.2005.08.001
State	Published - Oct 2005

Keywords

Glioma
Invasion
Migration
Rac
siRNA

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.ymeth.2005.08.001

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title = "Cell migration and invasion assays",

abstract = "The processes of cell migration and invasion are integral to embryonic development and the functioning of adult organisms. Deregulation of these processes contributes to numerous diseases. Ras GTPases and in particular members of the Rho subfamily of GTPases play critical roles in cell migration and invasion. Here, we provide a collection of protocols to assay these functions. We describe two cell migration assays. The monolayer wound healing assay is very easy to implement, whereas the microliter-scale migration assay allows examination of cell behavior on defined extracellular matrices. We also describe two methods that allow the quantification of tumor cell invasion, a versatile transwell Matrigel invasion assay and an organotypic assay that examines the invasion of glioma cells through a rat brain slice.",

keywords = "Glioma, Invasion, Migration, Rac, siRNA",

author = "Aline Valster and Tran, {Nhan L.} and Mitsutoshi Nakada and Berens, {Michael E.} and Chan, {Amanda Y.} and Marc Symons",

note = "Funding Information: This work was supported by National Institutes of Health Grant CA87567 to M.S. and NS042262 and NS043446 to M.E.B. ",

year = "2005",

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doi = "10.1016/j.ymeth.2005.08.001",

language = "English (US)",

volume = "37",

pages = "208--215",

journal = "Methods",

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publisher = "Academic Press Inc.",

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T1 - Cell migration and invasion assays

AU - Valster, Aline

AU - Tran, Nhan L.

AU - Nakada, Mitsutoshi

AU - Berens, Michael E.

AU - Chan, Amanda Y.

AU - Symons, Marc

N1 - Funding Information: This work was supported by National Institutes of Health Grant CA87567 to M.S. and NS042262 and NS043446 to M.E.B.

PY - 2005/10

Y1 - 2005/10

N2 - The processes of cell migration and invasion are integral to embryonic development and the functioning of adult organisms. Deregulation of these processes contributes to numerous diseases. Ras GTPases and in particular members of the Rho subfamily of GTPases play critical roles in cell migration and invasion. Here, we provide a collection of protocols to assay these functions. We describe two cell migration assays. The monolayer wound healing assay is very easy to implement, whereas the microliter-scale migration assay allows examination of cell behavior on defined extracellular matrices. We also describe two methods that allow the quantification of tumor cell invasion, a versatile transwell Matrigel invasion assay and an organotypic assay that examines the invasion of glioma cells through a rat brain slice.

AB - The processes of cell migration and invasion are integral to embryonic development and the functioning of adult organisms. Deregulation of these processes contributes to numerous diseases. Ras GTPases and in particular members of the Rho subfamily of GTPases play critical roles in cell migration and invasion. Here, we provide a collection of protocols to assay these functions. We describe two cell migration assays. The monolayer wound healing assay is very easy to implement, whereas the microliter-scale migration assay allows examination of cell behavior on defined extracellular matrices. We also describe two methods that allow the quantification of tumor cell invasion, a versatile transwell Matrigel invasion assay and an organotypic assay that examines the invasion of glioma cells through a rat brain slice.

KW - Glioma

KW - Invasion

KW - Migration

KW - Rac

KW - siRNA

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SN - 1046-2023

VL - 37

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JO - Methods

JF - Methods

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Cell migration and invasion assays

Abstract

Keywords

ASJC Scopus subject areas

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