Cell lineage in vascularized bone transplantation

Wouter F. Willems, Mikko Larsen, Patricia F. Friedrich, Allen Thorp Bishop

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background The biology behind vascularized bone allotransplantation remains largely unknown. We aim to study cell traffic between donor and recipient following bone auto-, and allografting. Methods Vascularized femoral transplantation was performed with arteriovenous bundle implantation and short-term immunosuppression. Twenty male Piebald Virol Glaxo (PVG; RT1 c) rats received isotransplants from female PVG (RT1c) rats and 22 male PVG rats received allografts from female Dark Agouti rats (DA, RT1a), representing a major histocompatibility mismatch. Both groups were randomly analyzed at 4 or 18 weeks. Bone remodeling areas (inner and outer cortical samples) were labeled and laser capture microdissected. Analysis of sex-mismatch genes by real-time reverse transcription-polymerase chain reaction provided the relative Expression Ratio (rER) of donor (female) to recipient (male) cells. Results The rER was 0.456 ± 0.266 at 4 weeks and 0.749 ± 0.387 at 18 weeks (p = 0.09) in allotransplants. In isotransplants, the rER was 0.412 ± 0.239 and 0.467 ± 0.252 at 4 and 18 weeks, respectively (p = 0.21). At 4 weeks, the rER at the outer cortical area of isotransplants was significantly lower in isotransplants as compared with allotransplants (0.247 ± 0.181 vs. 0.549 ± 0.184, p = 0.007). Cells in the inner and outer cortical bone remodeling areas in isotransplants were mainly donor derived (rER < 0.5) at 18 weeks, whereas allotransplants contained mainly recipient-derived cells (rER > 0.5) at 18 weeks. Conclusions Applying novel methodology, we describe detailed cell traffic in vascularized bone transplants, elaborating our comprehension on bone transplantation.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalMicrosurgery
Volume34
Issue number1
DOIs
StatePublished - Jan 2014

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Isografts
Bone Transplantation
Cell Lineage
Bone Remodeling
Bone and Bones
Histocompatibility
Autologous Transplantation
Homologous Transplantation
Thigh
Immunosuppression
Reverse Transcription
Allografts
Lasers
Transplantation
Transplants
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Surgery

Cite this

Cell lineage in vascularized bone transplantation. / Willems, Wouter F.; Larsen, Mikko; Friedrich, Patricia F.; Bishop, Allen Thorp.

In: Microsurgery, Vol. 34, No. 1, 01.2014, p. 37-43.

Research output: Contribution to journalArticle

Willems, WF, Larsen, M, Friedrich, PF & Bishop, AT 2014, 'Cell lineage in vascularized bone transplantation', Microsurgery, vol. 34, no. 1, pp. 37-43. https://doi.org/10.1002/micr.22147
Willems, Wouter F. ; Larsen, Mikko ; Friedrich, Patricia F. ; Bishop, Allen Thorp. / Cell lineage in vascularized bone transplantation. In: Microsurgery. 2014 ; Vol. 34, No. 1. pp. 37-43.
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