Abstract
Background The biology behind vascularized bone allotransplantation remains largely unknown. We aim to study cell traffic between donor and recipient following bone auto-, and allografting. Methods Vascularized femoral transplantation was performed with arteriovenous bundle implantation and short-term immunosuppression. Twenty male Piebald Virol Glaxo (PVG; RT1 c) rats received isotransplants from female PVG (RT1c) rats and 22 male PVG rats received allografts from female Dark Agouti rats (DA, RT1a), representing a major histocompatibility mismatch. Both groups were randomly analyzed at 4 or 18 weeks. Bone remodeling areas (inner and outer cortical samples) were labeled and laser capture microdissected. Analysis of sex-mismatch genes by real-time reverse transcription-polymerase chain reaction provided the relative Expression Ratio (rER) of donor (female) to recipient (male) cells. Results The rER was 0.456 ± 0.266 at 4 weeks and 0.749 ± 0.387 at 18 weeks (p = 0.09) in allotransplants. In isotransplants, the rER was 0.412 ± 0.239 and 0.467 ± 0.252 at 4 and 18 weeks, respectively (p = 0.21). At 4 weeks, the rER at the outer cortical area of isotransplants was significantly lower in isotransplants as compared with allotransplants (0.247 ± 0.181 vs. 0.549 ± 0.184, p = 0.007). Cells in the inner and outer cortical bone remodeling areas in isotransplants were mainly donor derived (rER < 0.5) at 18 weeks, whereas allotransplants contained mainly recipient-derived cells (rER > 0.5) at 18 weeks. Conclusions Applying novel methodology, we describe detailed cell traffic in vascularized bone transplants, elaborating our comprehension on bone transplantation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 37-43 |
| Number of pages | 7 |
| Journal | Microsurgery |
| Volume | 34 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2014 |
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ASJC Scopus subject areas
- Surgery
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Cell lineage in vascularized bone transplantation. / Willems, Wouter F.; Larsen, Mikko; Friedrich, Patricia F.; Bishop, Allen Thorp.
In: Microsurgery, Vol. 34, No. 1, 01.2014, p. 37-43.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell lineage in vascularized bone transplantation
AU - Willems, Wouter F.
AU - Larsen, Mikko
AU - Friedrich, Patricia F.
AU - Bishop, Allen Thorp
PY - 2014/1
Y1 - 2014/1
N2 - Background The biology behind vascularized bone allotransplantation remains largely unknown. We aim to study cell traffic between donor and recipient following bone auto-, and allografting. Methods Vascularized femoral transplantation was performed with arteriovenous bundle implantation and short-term immunosuppression. Twenty male Piebald Virol Glaxo (PVG; RT1 c) rats received isotransplants from female PVG (RT1c) rats and 22 male PVG rats received allografts from female Dark Agouti rats (DA, RT1a), representing a major histocompatibility mismatch. Both groups were randomly analyzed at 4 or 18 weeks. Bone remodeling areas (inner and outer cortical samples) were labeled and laser capture microdissected. Analysis of sex-mismatch genes by real-time reverse transcription-polymerase chain reaction provided the relative Expression Ratio (rER) of donor (female) to recipient (male) cells. Results The rER was 0.456 ± 0.266 at 4 weeks and 0.749 ± 0.387 at 18 weeks (p = 0.09) in allotransplants. In isotransplants, the rER was 0.412 ± 0.239 and 0.467 ± 0.252 at 4 and 18 weeks, respectively (p = 0.21). At 4 weeks, the rER at the outer cortical area of isotransplants was significantly lower in isotransplants as compared with allotransplants (0.247 ± 0.181 vs. 0.549 ± 0.184, p = 0.007). Cells in the inner and outer cortical bone remodeling areas in isotransplants were mainly donor derived (rER < 0.5) at 18 weeks, whereas allotransplants contained mainly recipient-derived cells (rER > 0.5) at 18 weeks. Conclusions Applying novel methodology, we describe detailed cell traffic in vascularized bone transplants, elaborating our comprehension on bone transplantation.
AB - Background The biology behind vascularized bone allotransplantation remains largely unknown. We aim to study cell traffic between donor and recipient following bone auto-, and allografting. Methods Vascularized femoral transplantation was performed with arteriovenous bundle implantation and short-term immunosuppression. Twenty male Piebald Virol Glaxo (PVG; RT1 c) rats received isotransplants from female PVG (RT1c) rats and 22 male PVG rats received allografts from female Dark Agouti rats (DA, RT1a), representing a major histocompatibility mismatch. Both groups were randomly analyzed at 4 or 18 weeks. Bone remodeling areas (inner and outer cortical samples) were labeled and laser capture microdissected. Analysis of sex-mismatch genes by real-time reverse transcription-polymerase chain reaction provided the relative Expression Ratio (rER) of donor (female) to recipient (male) cells. Results The rER was 0.456 ± 0.266 at 4 weeks and 0.749 ± 0.387 at 18 weeks (p = 0.09) in allotransplants. In isotransplants, the rER was 0.412 ± 0.239 and 0.467 ± 0.252 at 4 and 18 weeks, respectively (p = 0.21). At 4 weeks, the rER at the outer cortical area of isotransplants was significantly lower in isotransplants as compared with allotransplants (0.247 ± 0.181 vs. 0.549 ± 0.184, p = 0.007). Cells in the inner and outer cortical bone remodeling areas in isotransplants were mainly donor derived (rER < 0.5) at 18 weeks, whereas allotransplants contained mainly recipient-derived cells (rER > 0.5) at 18 weeks. Conclusions Applying novel methodology, we describe detailed cell traffic in vascularized bone transplants, elaborating our comprehension on bone transplantation.
UR - http://www.scopus.com/inward/record.url?scp=84891371331&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891371331&partnerID=8YFLogxK
U2 - 10.1002/micr.22147
DO - 10.1002/micr.22147
M3 - Article
C2 - 24038399
AN - SCOPUS:84891371331
VL - 34
SP - 37
EP - 43
JO - Microsurgery
JF - Microsurgery
SN - 0738-1085
IS - 1
ER -