Cell cycle independent interaction of Cdc2 with the centrosome, which is associated with the nuclear matrix-intermediate filament scaffold

Shirwin M. Pockwinse, Gabriela Krockmalnic, Stephen J. Doxsey, Jeffrey Nickerson, Jane B. Lian, Andre J van Wijnen, Janet L. Stein, Gary S. Stein, Sheldon Penman

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The cell cycle regulating Cdc2 protein kinase helps orchestrate cell cycle dependent changes in cell structure and function. This report shows that Cdc2 is localized to the centrosome region and is tightly bound to the nuclear matrix-intermediate filament scaffold. Antibodies to Cdc2 and to the centrosome-specific protein, pericentrin, label the centrosome in an apparently cell cycle independent manner. Isolated centrosomes also label similarly with both antibodies. Essentially, all cells show Cdc2 labeling of the centrosomes, implying an independence of the stage in the cell cycle, a conclusion supported by studies of synchronized cells. In contrast to the labeling of every cell with the Cdc2 monoclonal antibody, fewer centrosomes were labeled with an antibody to the PSTAIRE domain of Cdc2. Embedment-free, immunogold electron micrographs of extracted cell whole mounts show the centrioles and a pericentriolar network of filaments. Both Cdc2 and pericentrin antibodies decorate the amorphous pericentriolar material, while the Cdc2 antibodies also decorate the centrioles themselves. The constitutive presence of Cdc2 at the centrosome suggests a continuing role in the dynamics of centrosome function throughout the cell cycle.

Original languageEnglish (US)
Pages (from-to)3022-3027
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number7
DOIs
StatePublished - Apr 1 1997
Externally publishedYes

Fingerprint

Nuclear Matrix
Centrosome
Intermediate Filaments
Cell Cycle
Antibodies
Centrioles
CDC2 Protein Kinase
Protein Kinases
Monoclonal Antibodies
Electrons

Keywords

  • cyclin-dependent kinases
  • growth control
  • immunoelectron microscopy
  • osteoblasts
  • pericentrin

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Cell cycle independent interaction of Cdc2 with the centrosome, which is associated with the nuclear matrix-intermediate filament scaffold. / Pockwinse, Shirwin M.; Krockmalnic, Gabriela; Doxsey, Stephen J.; Nickerson, Jeffrey; Lian, Jane B.; van Wijnen, Andre J; Stein, Janet L.; Stein, Gary S.; Penman, Sheldon.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 7, 01.04.1997, p. 3022-3027.

Research output: Contribution to journalArticle

Pockwinse, Shirwin M. ; Krockmalnic, Gabriela ; Doxsey, Stephen J. ; Nickerson, Jeffrey ; Lian, Jane B. ; van Wijnen, Andre J ; Stein, Janet L. ; Stein, Gary S. ; Penman, Sheldon. / Cell cycle independent interaction of Cdc2 with the centrosome, which is associated with the nuclear matrix-intermediate filament scaffold. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 7. pp. 3022-3027.
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