Cell cycle-dependent inhibition of p34(cdc2) synthesis by transforming growth factor β1 in cycling epithelial cells

S. T. Eblen; M. P. Fautsch; R. J. Burnette; P. Joshi; E. B. Leof

Cell cycle-dependent inhibition of p34(cdc2) synthesis by transforming growth factor β₁ in cycling epithelial cells

S. T. Eblen, M. P. Fautsch, R. J. Burnette, P. Joshi, E. B. Leof

Research output: Contribution to journal › Article › peer-review

Abstract

Cycling epithelial cells were shown to reversibly arrest in late G₁ phase following treatment with transforming growth factor β₁. Associated with this G₁-S phase arrest was a decrease in the synthesis and histone H1 kinase activity of p34(cdc2). Transforming growth factor β₁ did not reduce p34(cdc2) levels by modulating the turnover of newly synthesized p34(cdc2). The decrease in p34(cdc2) synthesis preceded any detectable effect on DNA synthesis. Moreover, the action of transforming growth factor β₁ was regulated in a cell cycle-specific manner; epithelial cells were sensitive to transforming growth factor β₁ only during the G₁ phase. The results suggest that p34(cdc2) might be a useful biochemical marker for investigating the mechanism(s) of transforming growth factor β₁ signaling.

Original language	English (US)
Pages (from-to)	109-116
Number of pages	8
Journal	Cell Growth and Differentiation
Volume	5
Issue number	2
State	Published - 1994

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Cite this

@article{3819807605ea430daab63ea9dcd27abc,

title = "Cell cycle-dependent inhibition of p34(cdc2) synthesis by transforming growth factor β1 in cycling epithelial cells",

abstract = "Cycling epithelial cells were shown to reversibly arrest in late G1 phase following treatment with transforming growth factor β1. Associated with this G1-S phase arrest was a decrease in the synthesis and histone H1 kinase activity of p34(cdc2). Transforming growth factor β1 did not reduce p34(cdc2) levels by modulating the turnover of newly synthesized p34(cdc2). The decrease in p34(cdc2) synthesis preceded any detectable effect on DNA synthesis. Moreover, the action of transforming growth factor β1 was regulated in a cell cycle-specific manner; epithelial cells were sensitive to transforming growth factor β1 only during the G1 phase. The results suggest that p34(cdc2) might be a useful biochemical marker for investigating the mechanism(s) of transforming growth factor β1 signaling.",

author = "Eblen, {S. T.} and Fautsch, {M. P.} and Burnette, {R. J.} and P. Joshi and Leof, {E. B.}",

year = "1994",

language = "English (US)",

volume = "5",

pages = "109--116",

journal = "Cell Growth and Differentiation",

issn = "1044-9523",

publisher = "American Association for Cancer Research Inc.",

number = "2",

}

TY - JOUR

T1 - Cell cycle-dependent inhibition of p34(cdc2) synthesis by transforming growth factor β1 in cycling epithelial cells

AU - Eblen, S. T.

AU - Fautsch, M. P.

AU - Burnette, R. J.

AU - Joshi, P.

AU - Leof, E. B.

PY - 1994

Y1 - 1994

N2 - Cycling epithelial cells were shown to reversibly arrest in late G1 phase following treatment with transforming growth factor β1. Associated with this G1-S phase arrest was a decrease in the synthesis and histone H1 kinase activity of p34(cdc2). Transforming growth factor β1 did not reduce p34(cdc2) levels by modulating the turnover of newly synthesized p34(cdc2). The decrease in p34(cdc2) synthesis preceded any detectable effect on DNA synthesis. Moreover, the action of transforming growth factor β1 was regulated in a cell cycle-specific manner; epithelial cells were sensitive to transforming growth factor β1 only during the G1 phase. The results suggest that p34(cdc2) might be a useful biochemical marker for investigating the mechanism(s) of transforming growth factor β1 signaling.

AB - Cycling epithelial cells were shown to reversibly arrest in late G1 phase following treatment with transforming growth factor β1. Associated with this G1-S phase arrest was a decrease in the synthesis and histone H1 kinase activity of p34(cdc2). Transforming growth factor β1 did not reduce p34(cdc2) levels by modulating the turnover of newly synthesized p34(cdc2). The decrease in p34(cdc2) synthesis preceded any detectable effect on DNA synthesis. Moreover, the action of transforming growth factor β1 was regulated in a cell cycle-specific manner; epithelial cells were sensitive to transforming growth factor β1 only during the G1 phase. The results suggest that p34(cdc2) might be a useful biochemical marker for investigating the mechanism(s) of transforming growth factor β1 signaling.

UR - http://www.scopus.com/inward/record.url?scp=0028071664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028071664&partnerID=8YFLogxK

M3 - Article

C2 - 8180123

AN - SCOPUS:0028071664

SN - 1044-9523

VL - 5

SP - 109

EP - 116

JO - Cell Growth and Differentiation

JF - Cell Growth and Differentiation

IS - 2

ER -

Cell cycle-dependent inhibition of p34(cdc2) synthesis by transforming growth factor β₁ in cycling epithelial cells

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this