Cell-cell interaction in human granulopoiesis: Role of T lymphocytes

J. L. Ascensao, N. E. Kay, M. Banisadre, E. D. Zanjani

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

T lymphocytes have been implicated in the pathogenesis of granulocytopenias. We studied the effects of unstimulated and pokeweed mitogen (PWM) activated intact (Ti) and partially purified T cell subpopulations (Tγ and Tnonγ, i.e., Tμ plus T(0)) on in vitro granulocyte-macrophage colony formation (CFU(GM)) by autologous normal human bone marrow (BM). Coculture of BM with Ti, Tγ, or Tnonγ caused only a slight decrease in the numbers of colonies and clusters; however, when cultured with a mixture of Tγ and Tnonγ, the inhibition was significant. In contrast, activation of T cells or T cell subsets with PWM resulted in a marked decrease in colony formation. These results demonstrate that: 1) PWM-activated T cells or its subpopulations will inhibit autologous BM colony formation in vitro. The suppression seen with admixes of unstimulated Tγ and Tnonγ is presumptive evidence that prior cell-cell interaction(s) may be required for the generation of inhibitory cells in this system; and 2) this model may represent an in vitro counterpart of immune-mediated cytopenias in man.

Original languageEnglish (US)
Pages (from-to)473-478
Number of pages6
JournalExperimental Hematology
Volume9
Issue number5
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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    Ascensao, J. L., Kay, N. E., Banisadre, M., & Zanjani, E. D. (1981). Cell-cell interaction in human granulopoiesis: Role of T lymphocytes. Experimental Hematology, 9(5), 473-478.