C/EBPβ binds the P1 promoter of the Runx2 gene and up-regulates Runx2 transcription in osteoblastic cells

Berta Henriquez; Matias Hepp; Paola Merino; Hugo Sepulveda; Andre J. van Wijnen; Jane B. Lian; Gary S. Stein; Janet L. Stein; Martin Montecino

doi:10.1002/jcp.22652

C/EBPβ binds the P1 promoter of the Runx2 gene and up-regulates Runx2 transcription in osteoblastic cells

Berta Henriquez, Matias Hepp, Paola Merino, Hugo Sepulveda, Andre J. van Wijnen, Jane B. Lian, Gary S. Stein, Janet L. Stein, Martin Montecino

Orthopedic Surgery

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Abstract

The Runx2 factor is an essential component of the regulatory mechanisms that control transcription during skeletogenesis. Runx2/p57 expression in osteoblastic cells is controlled by the P1 promoter, which is recognized by key regulators of osteoblast differentiation including homeodomain factors and Wnt- and BMP-signaling mediators. Here, we report that the transcription factor C/EBPβ up-regulates Runx2/p57 expression by directly binding to the Runx2 P1 promoter in mesenchymal, pre-osteoblastic, and osteoblastic cells. This C/EBPβ-mediated up-regulation is principally dependent on C/EBP site II that is located within the first 180bp of the proximal P1 promoter region and is highly conserved among mouse, rat, and human Runx2 genes. Our studies reveal how the C/EBPβ factor, known to have a key role during osteogenesis, contributes to regulating the expression of Runx2, the master regulator of osteoblast differentiation.

Original language	English (US)
Pages (from-to)	3043-3052
Number of pages	10
Journal	Journal of Cellular Physiology
Volume	226
Issue number	11
DOIs	https://doi.org/10.1002/jcp.22652
State	Published - Nov 2011

ASJC Scopus subject areas

Physiology
Clinical Biochemistry
Cell Biology

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title = "C/EBPβ binds the P1 promoter of the Runx2 gene and up-regulates Runx2 transcription in osteoblastic cells",

abstract = "The Runx2 factor is an essential component of the regulatory mechanisms that control transcription during skeletogenesis. Runx2/p57 expression in osteoblastic cells is controlled by the P1 promoter, which is recognized by key regulators of osteoblast differentiation including homeodomain factors and Wnt- and BMP-signaling mediators. Here, we report that the transcription factor C/EBPβ up-regulates Runx2/p57 expression by directly binding to the Runx2 P1 promoter in mesenchymal, pre-osteoblastic, and osteoblastic cells. This C/EBPβ-mediated up-regulation is principally dependent on C/EBP site II that is located within the first 180bp of the proximal P1 promoter region and is highly conserved among mouse, rat, and human Runx2 genes. Our studies reveal how the C/EBPβ factor, known to have a key role during osteogenesis, contributes to regulating the expression of Runx2, the master regulator of osteoblast differentiation.",

author = "Berta Henriquez and Matias Hepp and Paola Merino and Hugo Sepulveda and {van Wijnen}, {Andre J.} and Lian, {Jane B.} and Stein, {Gary S.} and Stein, {Janet L.} and Martin Montecino",

year = "2011",

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doi = "10.1002/jcp.22652",

language = "English (US)",

volume = "226",

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AU - Henriquez, Berta

AU - Hepp, Matias

AU - Merino, Paola

AU - Sepulveda, Hugo

AU - van Wijnen, Andre J.

AU - Lian, Jane B.

AU - Stein, Gary S.

AU - Stein, Janet L.

AU - Montecino, Martin

PY - 2011/11

Y1 - 2011/11

N2 - The Runx2 factor is an essential component of the regulatory mechanisms that control transcription during skeletogenesis. Runx2/p57 expression in osteoblastic cells is controlled by the P1 promoter, which is recognized by key regulators of osteoblast differentiation including homeodomain factors and Wnt- and BMP-signaling mediators. Here, we report that the transcription factor C/EBPβ up-regulates Runx2/p57 expression by directly binding to the Runx2 P1 promoter in mesenchymal, pre-osteoblastic, and osteoblastic cells. This C/EBPβ-mediated up-regulation is principally dependent on C/EBP site II that is located within the first 180bp of the proximal P1 promoter region and is highly conserved among mouse, rat, and human Runx2 genes. Our studies reveal how the C/EBPβ factor, known to have a key role during osteogenesis, contributes to regulating the expression of Runx2, the master regulator of osteoblast differentiation.

AB - The Runx2 factor is an essential component of the regulatory mechanisms that control transcription during skeletogenesis. Runx2/p57 expression in osteoblastic cells is controlled by the P1 promoter, which is recognized by key regulators of osteoblast differentiation including homeodomain factors and Wnt- and BMP-signaling mediators. Here, we report that the transcription factor C/EBPβ up-regulates Runx2/p57 expression by directly binding to the Runx2 P1 promoter in mesenchymal, pre-osteoblastic, and osteoblastic cells. This C/EBPβ-mediated up-regulation is principally dependent on C/EBP site II that is located within the first 180bp of the proximal P1 promoter region and is highly conserved among mouse, rat, and human Runx2 genes. Our studies reveal how the C/EBPβ factor, known to have a key role during osteogenesis, contributes to regulating the expression of Runx2, the master regulator of osteoblast differentiation.

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JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

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ER -

C/EBPβ binds the P1 promoter of the Runx2 gene and up-regulates Runx2 transcription in osteoblastic cells

Abstract

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