cDNA cloning and characterization of a ligand for the Cek5 receptor protein-tyrosine kinase

Haining Shao, Liandi Lou, Akhilesh Pandey, Elena B. Pasquale, Vishva M. Dixit

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

We have isolated a murine cDNA encoding a ligand for the Cek5 receptor protein-tyrosine kinase (RPTK), a member of the Eph/Eck RPTK subfamily. Sequence analysis predicts an open reading frame of 345 amino acids with a predicted molecular mass of 38 kDa. Metabolic labeling and immunoprecipitation of cells transfected with a cDNA encoding the Cek5 ligand revealed the mature protein to have an apparent molecular mass of 45 kDa. The extracellular domain of the Cek5 ligand shows a 27% sequence identity at the protein level to B61, a ligand for the related Eck RPTK (Bartley, T. D., et al. (1994) Nature 368, 558-560). Consistent with the presence of a transmembrane domain, flow cytometry analysis revealed the Cek5 ligand to be expressed on the cell surface. The expressed Cek5 ligand is functionally active as it induces autophosphorylation of the Cek5 RPTK.

Original languageEnglish (US)
Pages (from-to)26606-26609
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number43
StatePublished - Oct 28 1994
Externally publishedYes

Fingerprint

Ephrin-B1
Cloning
Receptor Protein-Tyrosine Kinases
Organism Cloning
Complementary DNA
Molecular mass
Ephrin-A1
EphA1 Receptor
Flow cytometry
Immunoprecipitation
Protein-Tyrosine Kinases
Labeling
Open Reading Frames
Sequence Analysis
Flow Cytometry
Proteins
Ligands
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

cDNA cloning and characterization of a ligand for the Cek5 receptor protein-tyrosine kinase. / Shao, Haining; Lou, Liandi; Pandey, Akhilesh; Pasquale, Elena B.; Dixit, Vishva M.

In: Journal of Biological Chemistry, Vol. 269, No. 43, 28.10.1994, p. 26606-26609.

Research output: Contribution to journalArticle

Shao, Haining ; Lou, Liandi ; Pandey, Akhilesh ; Pasquale, Elena B. ; Dixit, Vishva M. / cDNA cloning and characterization of a ligand for the Cek5 receptor protein-tyrosine kinase. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 43. pp. 26606-26609.
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