cDNA and amino-acid sequences and organization of the gene encoding the Bβ subunit of fibrinogen from Xenopus laevis

Lewis Rowland Roberts, Lanita A. Nichols, LenéJ Holland

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fibrinogen, the major blood-clotting protein, is made up of three chains, Aα, Bβ and γ, which are synthesized and secreted by the liver. In this communication, we describe the complete cDNA sequence, deduced amino acid (aa) sequence and organization of the gene encoding the Bβ subunit of fibrinogen from Xenopus laevis (Xl). The cDNA representing the predominant form of the Bβ mRNA comprises 2390 nucleotides (nt), with an open reading frame of 1467 nt coding for a 488-aa protein. The percent identity between Xl Bβ and that of other animals ranges from 50% for lamprey to 66% for human. The Xl Bβ gene consists of nine exons, one more than found in the human gene. The exon/intron boundaries in the frog and human Bβ genes are in exactly conserved positions, except for junctions in the highly variable fibrinopeptide-encoding regions. Three of the exon/intron boundaries in the Xl Bβ gene are also analogous to ones in Aα and γ genes of other species, supporting the notion of a close evolutionary relationship between the genes for all three subunits. This analysis of Bβ from an amphibian provides the first complete description of the arrangement of exons and introns in any fibrinogen subunit gene from a non mammal and gives insight into the most highly conserved aspects of fibrinogen protein structure and gene organization.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalGene
Volume160
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Xenopus laevis
Fibrinogen
Amino Acid Sequence
Complementary DNA
Genes
Exons
Introns
Nucleotides
Lampreys
Blood Coagulation
Amphibians
Anura
Open Reading Frames
Blood Proteins
Mammals
Proteins
Amino Acids
Messenger RNA
Liver

Keywords

  • amphibian
  • Blood-clotting factor
  • exon/intron structure
  • fibrinogen sequence conservation
  • liver
  • molecular cloning

ASJC Scopus subject areas

  • Genetics

Cite this

cDNA and amino-acid sequences and organization of the gene encoding the Bβ subunit of fibrinogen from Xenopus laevis. / Roberts, Lewis Rowland; Nichols, Lanita A.; Holland, LenéJ.

In: Gene, Vol. 160, No. 2, 1995, p. 223-228.

Research output: Contribution to journalArticle

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