CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3′-end processing

Judith Pirngruber, Andrei Shchebet, Lisa Schreiber, Efrat Shema, Neri Minsky, Rob D. Chapman, Dirk Eick, Yael Aylon, Moshe Oren, Steven Johnsen

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Post-translational histone modifications have essential roles in controlling nuclear processes; however, the specific mechanisms regulating these modifications and their combinatorial activities remain elusive. Cyclin-dependent kinase 9 (CDK9) regulates gene expression by phosphorylating transcriptional regulatory proteins, including the RNA polymerase II carboxy-terminal domain. Here, we show that CDK9 activity is essential for maintaining global and gene-associated levels of histone H2B monoubiquitination (H2Bub1). Furthermore, CDK9 activity and H2Bub1 help to maintain correct replication-dependent histone messenger RNA (mRNA) 3′-end processing. CDK9 knockdown consistently resulted in inefficient recognition of the correct mRNA 3′-end cleavage site and led to increased read-through of RNA polymerase II to an alternative downstream polyadenylation signal. Thus, CDK9 acts to integrate phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing.

Original languageEnglish (US)
Pages (from-to)894-900
Number of pages7
JournalEMBO Reports
Volume10
Issue number8
DOIs
StatePublished - Jul 3 2009
Externally publishedYes

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RNA 3' End Processing
Cyclin-Dependent Kinase 9
Histones
Messenger RNA
Processing
RNA Polymerase II
Histone Code
Polyadenylation
Phosphorylation
Transcription
Post Translational Protein Processing
Gene expression
Chromatin
Genes
Gene Expression

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3′-end processing. / Pirngruber, Judith; Shchebet, Andrei; Schreiber, Lisa; Shema, Efrat; Minsky, Neri; Chapman, Rob D.; Eick, Dirk; Aylon, Yael; Oren, Moshe; Johnsen, Steven.

In: EMBO Reports, Vol. 10, No. 8, 03.07.2009, p. 894-900.

Research output: Contribution to journalArticle

Pirngruber, J, Shchebet, A, Schreiber, L, Shema, E, Minsky, N, Chapman, RD, Eick, D, Aylon, Y, Oren, M & Johnsen, S 2009, 'CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3′-end processing', EMBO Reports, vol. 10, no. 8, pp. 894-900. https://doi.org/10.1038/embor.2009.108
Pirngruber J, Shchebet A, Schreiber L, Shema E, Minsky N, Chapman RD et al. CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3′-end processing. EMBO Reports. 2009 Jul 3;10(8):894-900. https://doi.org/10.1038/embor.2009.108
Pirngruber, Judith ; Shchebet, Andrei ; Schreiber, Lisa ; Shema, Efrat ; Minsky, Neri ; Chapman, Rob D. ; Eick, Dirk ; Aylon, Yael ; Oren, Moshe ; Johnsen, Steven. / CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3′-end processing. In: EMBO Reports. 2009 ; Vol. 10, No. 8. pp. 894-900.
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