CD8+CD45RA+CCR7+FOXP3+ T cells with immunosuppressive properties: A novel subset of inducible human regulatory T cells

Masakatsu Suzuki, Ann L. Jagger, Christine Konya, Yasuhiro Shimojima, Sergey Pryshchep, Jörg J. Goronzy, Cornelia M. Weyand

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

CD8 T cells stimulated with a suboptimal dose of anti-CD3 Abs (100 pg/ml) in the presence of IL-15 retain a naive phenotype with expression of CD45RA, CD28, CD27, and CCR7 but acquire new functions and differentiate into immunosuppressive T cells. CD8+ CCR7+ regulatory T cells (Tregs) express FOXP3 and prevent CD4 T cells from responding to TCR stimulation and entering the cell cycle. Naive CD4 T cells are more susceptible to inhibition than memory cells. The suppressive activity of CD8 +CCR7+ Tregs is not mediated by IL-10, TGF-β, CTLA-4, CCL4, or adenosine and relies on interference with very early steps of the TCR signaling cascade. Specifically, CD8+CCR7+ Tregs prevent TCR-induced phosphorylation of ZAP70 and dampen the rise of intracellular calcium in CD4 T cells. The inducibility of CD8+CCR7+ Tregs is correlated with the age of the individual with PBLs of donors older than 60 y yielding low numbers of FOXP3low CD8 Tregs. Loss of CD8+CCR7+ Tregs in the elderly host may be of relevance in the aging immune system as immunosenescence is associated with a state of chronic smoldering inflammation.

Original languageEnglish (US)
Pages (from-to)2118-2130
Number of pages13
JournalJournal of Immunology
Volume189
Issue number5
DOIs
StatePublished - Sep 1 2012

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'CD8<sup>+</sup>CD45RA<sup>+</sup>CCR7<sup>+</sup>FOXP3<sup>+</sup> T cells with immunosuppressive properties: A novel subset of inducible human regulatory T cells'. Together they form a unique fingerprint.

Cite this