CD8 T cell-initiated vascular endothelial growth factor expression promotes central nervous system vascular permeability under neuroinflammatory conditions

Georgette L. Suidan, Jonathan W. Dickerson, Yi Chen, Jeremiah R. McDole, Pulak Tripathi, Istvan Pirko, Kim B. Seroogy, Aaron J. Johnson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Dysregulation of the blood-brain barrier (BBB) is a hallmark feature of numerous neurologic disorders as diverse as multiple sclerosis, stroke, epilepsy, viral hemorrhagic fevers, cerebral malaria, and acute hemorrhagic leukoencephalitis. CD8 T cells are one immune cell type that have been implicated in promoting vascular permeability in these conditions. Our laboratory has created a murine model of CD8 T cell-mediated CNS vascular permeability using a variation of the Theiler's murine encephalomyelitis virus system traditionally used to study multiple sclerosis. Previously, we demonstrated that CD8 T cells have the capacity to initiate astrocyte activation, cerebral endothelial cell tight junction protein alterations and CNS vascular permeability through a perforin-dependent process. To address the downstream mechanism by which CD8 T cells promote BBB dysregulation, in this study, we assess the role of vascular endothelial growth factor (VEGF) expression in this model. We demonstrate that neuronal expression of VEGF is significantly upregulated prior to, and coinciding with, CNS vascular permeability. Phosphorylation of fetal liver kinase-1 is significantly increased early in this process indicating activation of this receptor. Specific inhibition of neuropilin-1 significantly reduced CNS vascular permeability and fetal liver kinase-1 activation, and preserved levels of the cerebral endothelial cell tight junction protein occludin. Our data demonstrate that CD8 T cells initiate neuronal expression of VEGF in the CNS under neuroinflammatory conditions, and that VEGF may be a viable therapeutic target in neurologic disease characterized by inflammation-induced BBB disruption.

Original languageEnglish (US)
Pages (from-to)1031-1040
Number of pages10
JournalJournal of Immunology
Volume184
Issue number2
DOIs
StatePublished - Jan 15 2010
Externally publishedYes

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Capillary Permeability
Vascular Endothelial Growth Factor A
Central Nervous System
T-Lymphocytes
Blood-Brain Barrier
Tight Junction Proteins
Vascular Endothelial Growth Factor Receptor-2
Intercellular Junctions
Nervous System Diseases
Multiple Sclerosis
Acute Hemorrhagic Leukoencephalitis
Viral Hemorrhagic Fevers
Endothelial Cells
Neuropilin-1
Theilovirus
Occludin
Cerebral Malaria
Perforin
Astrocytes
Epilepsy

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

CD8 T cell-initiated vascular endothelial growth factor expression promotes central nervous system vascular permeability under neuroinflammatory conditions. / Suidan, Georgette L.; Dickerson, Jonathan W.; Chen, Yi; McDole, Jeremiah R.; Tripathi, Pulak; Pirko, Istvan; Seroogy, Kim B.; Johnson, Aaron J.

In: Journal of Immunology, Vol. 184, No. 2, 15.01.2010, p. 1031-1040.

Research output: Contribution to journalArticle

Suidan, Georgette L. ; Dickerson, Jonathan W. ; Chen, Yi ; McDole, Jeremiah R. ; Tripathi, Pulak ; Pirko, Istvan ; Seroogy, Kim B. ; Johnson, Aaron J. / CD8 T cell-initiated vascular endothelial growth factor expression promotes central nervous system vascular permeability under neuroinflammatory conditions. In: Journal of Immunology. 2010 ; Vol. 184, No. 2. pp. 1031-1040.
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