CD40 mediates downregulation of CD32B on specific memory B cell populations in rheumatoid arthritis

Xiaoyu Zhang, Erin Burch, Ling Cai, Edward So, Fleesie Hubbard, Eric Lawrence Matteson, Scott E. Strome

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Altered B cell function is important in the pathogenesis of rheumatoid arthritis (RA). In this report, we show that patients with active RA have an increased frequency of CD32B low/neg cells in the CD27+IgD - memory B cell subset and that these changes are associated with phenotypic and functional B cell activation. Studies using PBMCs from healthy donors revealed that downregulation of CD32B on B cells is mediated by CD40-CD40L interactions and is potentiated by IL-4 and inhibited by both IL-10 and IL-21. These findings appear physiologically relevant because CD4 T cell expression of CD40L correlated with the frequency of CD32B low/neg cells in the CD27+IgD- memory B subset in patients with RA. Our data support a model in which high levels of CD40L, present on circulating T cells in patients with RA, causes B cell activation and CD32B downregulation, resulting in secondary protection of memory B cells from CD32B-mediated cell death.

Original languageEnglish (US)
Pages (from-to)6015-6022
Number of pages8
JournalJournal of Immunology
Volume190
Issue number12
DOIs
StatePublished - Jun 15 2013

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Rheumatoid Arthritis
B-Lymphocytes
Down-Regulation
CD40 Ligand
Immunoglobulin D
Population
B-Lymphocyte Subsets
T-Lymphocytes
Interleukin-4
Interleukin-10
Cell Death
Tissue Donors

ASJC Scopus subject areas

  • Immunology

Cite this

CD40 mediates downregulation of CD32B on specific memory B cell populations in rheumatoid arthritis. / Zhang, Xiaoyu; Burch, Erin; Cai, Ling; So, Edward; Hubbard, Fleesie; Matteson, Eric Lawrence; Strome, Scott E.

In: Journal of Immunology, Vol. 190, No. 12, 15.06.2013, p. 6015-6022.

Research output: Contribution to journalArticle

Zhang, Xiaoyu ; Burch, Erin ; Cai, Ling ; So, Edward ; Hubbard, Fleesie ; Matteson, Eric Lawrence ; Strome, Scott E. / CD40 mediates downregulation of CD32B on specific memory B cell populations in rheumatoid arthritis. In: Journal of Immunology. 2013 ; Vol. 190, No. 12. pp. 6015-6022.
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AU - Matteson, Eric Lawrence

AU - Strome, Scott E.

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