CD38-NADase is a new major contributor to Duchenne muscular dystrophic phenotype

Antoine de Zélicourt, Abdallah Fayssoil, Mbarka Dakouane-Giudicelli, Isley De Jesus, Ahmed Karoui, Faouzi Zarrouki, Florence Lefebvre, Arnaud Mansart, Jean Marie Launay, Jerome Piquereau, Mariana G. Tarragó, Marcel Bonay, Anne Forand, Sophie Moog, France Piétri-Rouxel, Elise Brisebard, Claudia C.S. Chini, Sonu Kashyap, Matthew J. Fogarty, Gary C. SieckMathias Mericskay, Eduardo N. Chini, Ana Maria Gomez, José Manuel Cancela, Sabine de la Porte

Research output: Contribution to journalArticlepeer-review


Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration. Two important deleterious features are a Ca2+ dysregulation linked to Ca2+ influxes associated with ryanodine receptor hyperactivation, and a muscular nicotinamide adenine dinucleotide (NAD+) deficit. Here, we identified that deletion in mdx mice of CD38, a NAD+ glycohydrolase-producing modulators of Ca2+ signaling, led to a fully restored heart function and structure, with skeletal muscle performance improvements, associated with a reduction in inflammation and senescence markers. Muscle NAD+ levels were also fully restored, while the levels of the two main products of CD38, nicotinamide and ADP-ribose, were reduced, in heart, diaphragm, and limb. In cardiomyocytes from mdx/CD38−/− mice, the pathological spontaneous Ca2+ activity was reduced, as well as in myotubes from DMD patients treated with isatuximab (SARCLISA®) a monoclonal anti-CD38 antibody. Finally, treatment of mdx and utrophin–dystrophin-deficient (mdx/utr−/−) mice with CD38 inhibitors resulted in improved skeletal muscle performances. Thus, we demonstrate that CD38 actively contributes to DMD physiopathology. We propose that a selective anti-CD38 therapeutic intervention could be highly relevant to develop for DMD patients.

Original languageEnglish (US)
Article numbere12860
JournalEMBO Molecular Medicine
Issue number5
StatePublished - May 9 2022


  • CD38
  • DMD
  • NAD
  • calcium
  • cardiomyopathy

ASJC Scopus subject areas

  • Molecular Medicine


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