CD38 expression, function, and gene resequencing in a human lymphoblastoid cell line-based model system

William R. Hartman, Linda L. Pelleymounter, Irene Moon, Krishna R Kalari, Mohan Liu, Tse Yu Wu, Carlos Escande, Veronica Nin, Eduardo Nunes Chini, Richard M Weinshilboum

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

CD38 is an ecto-enzyme that hydrolyzes NAD. Its expression is a prognostic marker for chronic lymphocytic leukemia. We have characterized individual variation in CD38 expression in lymphoblastoid cell lines from 288 healthy subjects of three ethnicities. Expression varied widely, with significant differences among ethnic groups, and was correlated significantly with CD38 enzymatic activity and protein levels. The CD38 gene was then resequenced using DNA from the same cell lines, with the identification of 53 single nucleotide polymorphisms (SNPs) and one indel, 39 novel. One SNP, rs1130169, was significantly associated with CD38 mRNA expression and explained a portion of the difference in expression among ethnic groups. EMS assay showed nuclear protein binding at or near this SNP. We also determined that variation in CD38 expression in these cell lines was associated with variation in antineoplastic drug sensitivity. These results represent a step toward understanding mechanisms involved in CD38 expression.

Original languageEnglish (US)
Pages (from-to)1315-1325
Number of pages11
JournalLeukemia and Lymphoma
Volume51
Issue number7
DOIs
StatePublished - Jul 2010

Fingerprint

Single Nucleotide Polymorphism
Gene Expression
Ethnic Groups
Cell Line
B-Cell Chronic Lymphocytic Leukemia
Nuclear Proteins
Protein Binding
Antineoplastic Agents
NAD
Healthy Volunteers
Messenger RNA
DNA
Enzymes
Genes
Proteins

Keywords

  • CD38
  • expression variation
  • gene expression
  • microarray
  • mRNA expression
  • NAD
  • polymorphism
  • single nucleotide polymorphisms
  • SNPs

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

CD38 expression, function, and gene resequencing in a human lymphoblastoid cell line-based model system. / Hartman, William R.; Pelleymounter, Linda L.; Moon, Irene; Kalari, Krishna R; Liu, Mohan; Wu, Tse Yu; Escande, Carlos; Nin, Veronica; Chini, Eduardo Nunes; Weinshilboum, Richard M.

In: Leukemia and Lymphoma, Vol. 51, No. 7, 07.2010, p. 1315-1325.

Research output: Contribution to journalArticle

Hartman, William R. ; Pelleymounter, Linda L. ; Moon, Irene ; Kalari, Krishna R ; Liu, Mohan ; Wu, Tse Yu ; Escande, Carlos ; Nin, Veronica ; Chini, Eduardo Nunes ; Weinshilboum, Richard M. / CD38 expression, function, and gene resequencing in a human lymphoblastoid cell line-based model system. In: Leukemia and Lymphoma. 2010 ; Vol. 51, No. 7. pp. 1315-1325.
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AU - Wu, Tse Yu

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