CD34+ CD38- and CD34+ HLA-DR- cells in BM stem cell grafts correlate with short-term engraftment but have no influence on long-term hematopoietic reconstitution after autologous transplantation

Abba Zubair, G. Kao, H. Daley, D. Schott, A. Freedman, J. Ritz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Prior studies have demonstrated that relatively immature hematopoietic stem cells, including CD34+ CD38- and CD34+ HLA-DR- subsets, correlate with short-term hematopoietic reconstruction (SHR) after transplantation. The aim of this study was to investigate whether these immature CD34+ subsets also correlate with long-term hematopoietic reconstitution (LHR) in recipients of ABMT. Methods: We examined stem cell grafts from 58 patients with B-cell lymphoma or CLL who underwent ABMT after myeloablative conditioning. We determined whether total mononuclear cell dose (MNC), colony-forming unit-granulocyte-monocyte (CFU-GM), CD34+ cell dose and CD34+ cell subsets (CD34+ CD38- and CD34+ HLA-DR-) were associated with SHR and/or LHR. Time to neutrophil engraftment (TNE) and time to platelet engraftment (TPE) were used to measure SHR, while platelet counts at day 100 and 1 year post-ABMT were used as indicators for LHR. Results and discussion: CD34+ cell dose and CD34+ cell subsets were significantly associated with SHR. However, at day 100 and 1 year post-transplant only total CD34+ cell dose was associated with LHR. The association of total CD34+ cell dose with LHR persisted after adjusting for age, sex and disease. None of the CD34+ cell subsets analyzed showed evidence of significant association with LHR.

Original languageEnglish (US)
Pages (from-to)399-407
Number of pages9
JournalCytotherapy
Volume8
Issue number4
DOIs
StatePublished - Aug 2006

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Autologous Transplantation
HLA-DR Antigens
Stem Cells
Transplants
B-Cell Lymphoma
Hematopoietic Stem Cells
Platelet Count
Granulocytes
Monocytes
Neutrophils
Blood Platelets
Transplantation

Keywords

  • ABMT
  • CD34
  • Engraftment
  • Leukemia
  • Lymphoma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Cancer Research
  • Transplantation

Cite this

CD34+ CD38- and CD34+ HLA-DR- cells in BM stem cell grafts correlate with short-term engraftment but have no influence on long-term hematopoietic reconstitution after autologous transplantation. / Zubair, Abba; Kao, G.; Daley, H.; Schott, D.; Freedman, A.; Ritz, J.

In: Cytotherapy, Vol. 8, No. 4, 08.2006, p. 399-407.

Research output: Contribution to journalArticle

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abstract = "Background: Prior studies have demonstrated that relatively immature hematopoietic stem cells, including CD34+ CD38- and CD34+ HLA-DR- subsets, correlate with short-term hematopoietic reconstruction (SHR) after transplantation. The aim of this study was to investigate whether these immature CD34+ subsets also correlate with long-term hematopoietic reconstitution (LHR) in recipients of ABMT. Methods: We examined stem cell grafts from 58 patients with B-cell lymphoma or CLL who underwent ABMT after myeloablative conditioning. We determined whether total mononuclear cell dose (MNC), colony-forming unit-granulocyte-monocyte (CFU-GM), CD34+ cell dose and CD34+ cell subsets (CD34+ CD38- and CD34+ HLA-DR-) were associated with SHR and/or LHR. Time to neutrophil engraftment (TNE) and time to platelet engraftment (TPE) were used to measure SHR, while platelet counts at day 100 and 1 year post-ABMT were used as indicators for LHR. Results and discussion: CD34+ cell dose and CD34+ cell subsets were significantly associated with SHR. However, at day 100 and 1 year post-transplant only total CD34+ cell dose was associated with LHR. The association of total CD34+ cell dose with LHR persisted after adjusting for age, sex and disease. None of the CD34+ cell subsets analyzed showed evidence of significant association with LHR.",
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