CD33 detection by immunohistochemistry in paraffin-embedded tissues: A new antibody shows excellent specificity and sensitivity for cells of myelomonocytic lineage

James D. Hoyer, Karen L. Grogg, Curtis A. Hanson, Jeffrey D. Gamez, Ahmet Dogan

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

A new monoclonal antibody to CD33 that reacts in paraffin-embedded tissue samples was evaluated. The expected reactivity in granulocytic and monocytic cells was found in a tissue microarray composed of multiple tissue sites. There was no unexpected reactivity found in a wide variety of hematolymphoid and nonhematolymphoid disorders. In cases of acute leukemia, the CD33 antibody showed equivalent results by immunohistochemical analysis compared with flow cytometric analysis. The CD33 antibody was also found to be a useful marker in the workup of myeloid sarcomas. This anti-CD33 antibody will be a useful marker in the workup of acute leukemias and myeloid sarcomas on paraffin-embedded tissue samples.

Original languageEnglish (US)
Pages (from-to)316-323
Number of pages8
JournalAmerican journal of clinical pathology
Volume129
Issue number2
DOIs
StatePublished - Feb 1 2008

Keywords

  • Acute leukemia
  • CD33
  • Flow cytometry
  • Immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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