CD24hiCD38hi and CD24hiCD27+ Human Regulatory B Cells Display Common and Distinct Functional Characteristics

Md Mahmudul Hasan, Lu Ann Thompson-Snipes, Goran Klintmalm, Anthony J. Demetris, Jacqueline O'Leary, Sang Kon Oh, Hye Mee Joo

Research output: Contribution to journalArticle

Abstract

Although IL-10-producing regulatory B cells (Bregs) play important roles in immune regulation, their surface phenotypes and functional characteristics have not been fully investigated. In this study, we report that the frequency of IL-10-producing Bregs in human peripheral blood, spleens, and tonsils is similar, but they display heterogenous surface phenotypes. Nonetheless, CD24hiCD38hi transitional B cells (TBs) and CD24hiCD27+ B cells (human equivalent of murine B10 cells) are the major IL-10-producing B cells. They both suppress CD4+ T cell proliferation as well as IFN-γ/IL-17 expression. However, CD24hiCD27+ B cells were more efficient than TBs at suppressing CD4+ T cell proliferation and IFN-γ/IL-17 expression, whereas they both coexpress IL-10 and TNF-α. TGF-β1 and granzyme B expression were also enriched within CD24hiCD27+ B cells, when compared with TBs. Additionally, CD24hiCD27+ B cells expressed increased levels of surface integrins (CD11a, CD11b, α1, α4, and β1) and CD39 (an ecto-ATPase), suggesting that the in vivo mechanisms of action of the two Breg subsets are not the same. Lastly, we also report that liver allograft recipients with plasma cell hepatitis had significant decreases of both Breg subsets.

Original languageEnglish (US)
Pages (from-to)2110-2120
Number of pages11
JournalJournal of immunology (Baltimore, Md. : 1950)
Volume203
Issue number8
DOIs
StatePublished - Oct 15 2019

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Regulatory B-Lymphocytes
B-Lymphocytes
Interleukin-10
Interleukin-17
Cell Proliferation
T-Lymphocytes
Phenotype
Granzymes
B-Lymphoid Precursor Cells
Palatine Tonsil
Plasma Cells
Integrins
Hepatitis
Allografts
Spleen
Liver

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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CD24hiCD38hi and CD24hiCD27+ Human Regulatory B Cells Display Common and Distinct Functional Characteristics. / Hasan, Md Mahmudul; Thompson-Snipes, Lu Ann; Klintmalm, Goran; Demetris, Anthony J.; O'Leary, Jacqueline; Oh, Sang Kon; Joo, Hye Mee.

In: Journal of immunology (Baltimore, Md. : 1950), Vol. 203, No. 8, 15.10.2019, p. 2110-2120.

Research output: Contribution to journalArticle

Hasan, Md Mahmudul ; Thompson-Snipes, Lu Ann ; Klintmalm, Goran ; Demetris, Anthony J. ; O'Leary, Jacqueline ; Oh, Sang Kon ; Joo, Hye Mee. / CD24hiCD38hi and CD24hiCD27+ Human Regulatory B Cells Display Common and Distinct Functional Characteristics. In: Journal of immunology (Baltimore, Md. : 1950). 2019 ; Vol. 203, No. 8. pp. 2110-2120.
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