CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn's disease

Konstantinos A. Papadakis; John Prehn; Sofia T. Moreno; Lorna Cheng; Elias A. Kouroumalis; Richard Deem; Tim Breaverman; Paul D. Ponath; David P. Andrew; Peter H.R. Green; Martin R. Hodge; Scott W. Binder; Stephan R. Targan

doi:10.1053/gast.2001.27154

CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn's disease

Konstantinos A. Papadakis, John Prehn, Sofia T. Moreno, Lorna Cheng, Elias A. Kouroumalis, Richard Deem, Tim Breaverman, Paul D. Ponath, David P. Andrew, Peter H.R. Green, Martin R. Hodge, Scott W. Binder, Stephan R. Targan

Gastroenterology and Hepatology

Research output: Contribution to journal › Article › peer-review

170 Scopus citations

Abstract

Background & Aims: Thymus-expressed chemokine (TECK) or CCL25) is selectively expressed in the small bowel (SB), where lamina propria lymphocytes (LPL) and intraepithelial leukocyte expressing the cognate chemokine receptor CCR9 predominate. We characterize the role of TECK and CCR9-expresing lymphocytes in small intestinal Crohn's disease. Methods: CCR9 expression on lymphocytes from lamina propria, mesenteric lymph node, and peripheral blood was analyzed by flow cytometry and by Northern blotting for LPL. TECK expression was analyzed in inflamed SB and colon by reverse-transcription polymerase chain reaction and immunohistochemistry. Results: The fraction of CCR9⁺ T cells in inflamed SB was significantly lower than in uninvolved SB mucosa. In contrast, in peripheral blood lymphocytes, CCR9⁺ lymphocytes were markedly elevated in patients with small bowel Crohn's or celiac disease, but not in patients with purely colonic Crohn's. Also, TECK expression is altered in inflamed small bowel, being intensely expressed in a patchy distribution in crypt epithelial cells in proximity to lymphocytic infiltrates. TECK is not expressed in either normal or inflamed colon. Conclusions: In SB immune-mediated diseases, there is repartitioning of CCR9⁺ lymphocytes between SB and blood and an altered pattern of TECK expression in SB Crohn's. The TECK/CCR9 ligand/receptor pair may play an important role in the pathogenesis of SB Crohn's disease.

Original language	English (US)
Pages (from-to)	246-254
Number of pages	9
Journal	Gastroenterology
Volume	121
Issue number	2
DOIs	https://doi.org/10.1053/gast.2001.27154
State	Published - 2001

ASJC Scopus subject areas

Hepatology
Gastroenterology

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10.1053/gast.2001.27154

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Papadakis, K. A., Prehn, J., Moreno, S. T., Cheng, L., Kouroumalis, E. A., Deem, R., Breaverman, T., Ponath, P. D., Andrew, D. P., Green, P. H. R., Hodge, M. R., Binder, S. W., & Targan, S. R. (2001). CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn's disease. Gastroenterology, 121(2), 246-254. https://doi.org/10.1053/gast.2001.27154

@article{c97ab9c156234ad4ab2d74ad76be4765,

title = "CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn's disease",

abstract = "Background & Aims: Thymus-expressed chemokine (TECK) or CCL25) is selectively expressed in the small bowel (SB), where lamina propria lymphocytes (LPL) and intraepithelial leukocyte expressing the cognate chemokine receptor CCR9 predominate. We characterize the role of TECK and CCR9-expresing lymphocytes in small intestinal Crohn's disease. Methods: CCR9 expression on lymphocytes from lamina propria, mesenteric lymph node, and peripheral blood was analyzed by flow cytometry and by Northern blotting for LPL. TECK expression was analyzed in inflamed SB and colon by reverse-transcription polymerase chain reaction and immunohistochemistry. Results: The fraction of CCR9+ T cells in inflamed SB was significantly lower than in uninvolved SB mucosa. In contrast, in peripheral blood lymphocytes, CCR9+ lymphocytes were markedly elevated in patients with small bowel Crohn's or celiac disease, but not in patients with purely colonic Crohn's. Also, TECK expression is altered in inflamed small bowel, being intensely expressed in a patchy distribution in crypt epithelial cells in proximity to lymphocytic infiltrates. TECK is not expressed in either normal or inflamed colon. Conclusions: In SB immune-mediated diseases, there is repartitioning of CCR9+ lymphocytes between SB and blood and an altered pattern of TECK expression in SB Crohn's. The TECK/CCR9 ligand/receptor pair may play an important role in the pathogenesis of SB Crohn's disease.",

author = "Papadakis, {Konstantinos A.} and John Prehn and Moreno, {Sofia T.} and Lorna Cheng and Kouroumalis, {Elias A.} and Richard Deem and Tim Breaverman and Ponath, {Paul D.} and Andrew, {David P.} and Green, {Peter H.R.} and Hodge, {Martin R.} and Binder, {Scott W.} and Targan, {Stephan R.}",

year = "2001",

doi = "10.1053/gast.2001.27154",

language = "English (US)",

volume = "121",

pages = "246--254",

journal = "Gastroenterology",

issn = "0016-5085",

publisher = "W.B. Saunders Ltd",

number = "2",

}

TY - JOUR

T1 - CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn's disease

AU - Papadakis, Konstantinos A.

AU - Prehn, John

AU - Moreno, Sofia T.

AU - Cheng, Lorna

AU - Kouroumalis, Elias A.

AU - Deem, Richard

AU - Breaverman, Tim

AU - Ponath, Paul D.

AU - Andrew, David P.

AU - Green, Peter H.R.

AU - Hodge, Martin R.

AU - Binder, Scott W.

AU - Targan, Stephan R.

PY - 2001

Y1 - 2001

N2 - Background & Aims: Thymus-expressed chemokine (TECK) or CCL25) is selectively expressed in the small bowel (SB), where lamina propria lymphocytes (LPL) and intraepithelial leukocyte expressing the cognate chemokine receptor CCR9 predominate. We characterize the role of TECK and CCR9-expresing lymphocytes in small intestinal Crohn's disease. Methods: CCR9 expression on lymphocytes from lamina propria, mesenteric lymph node, and peripheral blood was analyzed by flow cytometry and by Northern blotting for LPL. TECK expression was analyzed in inflamed SB and colon by reverse-transcription polymerase chain reaction and immunohistochemistry. Results: The fraction of CCR9+ T cells in inflamed SB was significantly lower than in uninvolved SB mucosa. In contrast, in peripheral blood lymphocytes, CCR9+ lymphocytes were markedly elevated in patients with small bowel Crohn's or celiac disease, but not in patients with purely colonic Crohn's. Also, TECK expression is altered in inflamed small bowel, being intensely expressed in a patchy distribution in crypt epithelial cells in proximity to lymphocytic infiltrates. TECK is not expressed in either normal or inflamed colon. Conclusions: In SB immune-mediated diseases, there is repartitioning of CCR9+ lymphocytes between SB and blood and an altered pattern of TECK expression in SB Crohn's. The TECK/CCR9 ligand/receptor pair may play an important role in the pathogenesis of SB Crohn's disease.

AB - Background & Aims: Thymus-expressed chemokine (TECK) or CCL25) is selectively expressed in the small bowel (SB), where lamina propria lymphocytes (LPL) and intraepithelial leukocyte expressing the cognate chemokine receptor CCR9 predominate. We characterize the role of TECK and CCR9-expresing lymphocytes in small intestinal Crohn's disease. Methods: CCR9 expression on lymphocytes from lamina propria, mesenteric lymph node, and peripheral blood was analyzed by flow cytometry and by Northern blotting for LPL. TECK expression was analyzed in inflamed SB and colon by reverse-transcription polymerase chain reaction and immunohistochemistry. Results: The fraction of CCR9+ T cells in inflamed SB was significantly lower than in uninvolved SB mucosa. In contrast, in peripheral blood lymphocytes, CCR9+ lymphocytes were markedly elevated in patients with small bowel Crohn's or celiac disease, but not in patients with purely colonic Crohn's. Also, TECK expression is altered in inflamed small bowel, being intensely expressed in a patchy distribution in crypt epithelial cells in proximity to lymphocytic infiltrates. TECK is not expressed in either normal or inflamed colon. Conclusions: In SB immune-mediated diseases, there is repartitioning of CCR9+ lymphocytes between SB and blood and an altered pattern of TECK expression in SB Crohn's. The TECK/CCR9 ligand/receptor pair may play an important role in the pathogenesis of SB Crohn's disease.

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DO - 10.1053/gast.2001.27154

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SN - 0016-5085

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SP - 246

EP - 254

JO - Gastroenterology

JF - Gastroenterology

IS - 2

ER -

CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn's disease

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