Caveolin-2 localizes to the Golgi complex but redistributes to plasma membrane, caveolae, and rafts when co-expressed with caveolin-1

Rosalia Mora, Vera L. Bonilha, Alan D Marmorstein, Philipp E. Scherer, Dennis Brown, Michael P. Lisanti, Enrique Rodriguez-Boulan

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

We have characterized comparatively the subcellular distributions of caveolins-1 and -2, their interactions and their roles in caveolar formation in polarized epithelial cells. In Fischer rat thyroid (FRT) cells, which express low levels of caveolin-2 and no caveolin-1, caveolin-2 localizes exclusively to the Golgi complex but is partially redistributed to the plasma membrane upon co-expression of caveolin-1 by transfection or by adenovirus- mediated transduction. In Madin-Darby canine kidney (MDCK) cells, which constitutively express both caveolin-1 and -2, caveolin-2 localized to both the Golgi complex and to the plasma membrane, where it co-distributed with caveolin-1 in flat patches and in caveolae. In FRT cells, endogenous or overexpressed caveolin-2 did not associate with low density Triton insoluble membranes that floated in sucrose density gradients but was recruited to these membranes when co-expressed together with caveolin-1. In MDCK cells, both caveolin-1 and caveolin-2 associated with low density Triton-insoluble membranes. In FRT cells, transfection of caveolin-1 promoted the assembly of plasma membrane caveolae that localized preferentially (over 99%) to the basolateral surface, like constitutive caveolae of MDCK cells. In contrast, as expected from its intracellular distribution, endogenous or overexpressed caveolin-2 did not promote the assembly of caveolae; rather, it appeared to promote the assembly of intracellular vesicles in the peri-Golgi area. The data reported here demonstrate that caveolin-1 and -2 have different and complementary subcellular localizations and functional properties in polarized epithelial cells and suggest that the two proteins co-operate to carry out specific as yet unknown tasks between the Golgi complex and the cell surface.

Original languageEnglish (US)
Pages (from-to)25708-25717
Number of pages10
JournalJournal of Biological Chemistry
Volume274
Issue number36
DOIs
StatePublished - Sep 3 1999
Externally publishedYes

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Caveolin 2
Caveolin 1
Caveolae
Golgi Apparatus
Cell membranes
Cell Membrane
Madin Darby Canine Kidney Cells
Inbred F344 Rats
Rats
Thyroid Gland
Membranes
Transfection
Epithelial Cells
Adenoviridae
Sucrose

ASJC Scopus subject areas

  • Biochemistry

Cite this

Caveolin-2 localizes to the Golgi complex but redistributes to plasma membrane, caveolae, and rafts when co-expressed with caveolin-1. / Mora, Rosalia; Bonilha, Vera L.; Marmorstein, Alan D; Scherer, Philipp E.; Brown, Dennis; Lisanti, Michael P.; Rodriguez-Boulan, Enrique.

In: Journal of Biological Chemistry, Vol. 274, No. 36, 03.09.1999, p. 25708-25717.

Research output: Contribution to journalArticle

Mora, Rosalia ; Bonilha, Vera L. ; Marmorstein, Alan D ; Scherer, Philipp E. ; Brown, Dennis ; Lisanti, Michael P. ; Rodriguez-Boulan, Enrique. / Caveolin-2 localizes to the Golgi complex but redistributes to plasma membrane, caveolae, and rafts when co-expressed with caveolin-1. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 36. pp. 25708-25717.
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