CatroxMP-II

a heme-modulated fibrinogenolytic metalloproteinase isolated from Crotalus atrox venom

Montamas Suntravat, Paul R Langlais, Elda E. Sánchez, Vance G. Nielsen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

It has been recently demonstrated that the hemotoxic venom activity of several species of snakes can be inhibited by carbon monoxide (CO) or a metheme forming agent. These and other data suggest that the biometal, heme, may be attached to venom enzymes and may be modulated by CO. A novel fibrinogenolytic metalloproteinase, named CatroxMP-II, was isolated and purified from the venom of a Crotalus atrox viper, and subjected to proteolysis and mass spectroscopy. An ion similar to the predicted singly charged m/z of heme at 617.18 was identified. Lastly, CORM-2 (tricarbonyldichlororuthenium (II) dimer, a CO releasing molecule) inhibited the fibrinogenolytic effects of CatroxMP-II on coagulation kinetics in human plasma. In conclusion, we present the first example of a snake venom metalloproteinase that is heme-bound and CO-inhibited.

Original languageEnglish (US)
Pages (from-to)585-593
Number of pages9
JournalBioMetals
Volume31
Issue number4
DOIs
StatePublished - Aug 1 2018
Externally publishedYes

Fingerprint

atrolysin A
Crotalus atrox
carbon monoxide
Venoms
venoms
Metalloproteases
metalloproteinases
heme
Carbon Monoxide
Heme
Carbon monoxide
snakes
Proteolysis
Crotalus
Plasma (human)
Viperidae
Snake Venoms
Snakes
Trace Elements
Coagulation

Keywords

  • Carbon monoxide
  • Heme
  • Mass spectrometry
  • Snake venom metalloproteinase
  • Thrombelastography

ASJC Scopus subject areas

  • Biomaterials
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Metals and Alloys

Cite this

CatroxMP-II : a heme-modulated fibrinogenolytic metalloproteinase isolated from Crotalus atrox venom. / Suntravat, Montamas; Langlais, Paul R; Sánchez, Elda E.; Nielsen, Vance G.

In: BioMetals, Vol. 31, No. 4, 01.08.2018, p. 585-593.

Research output: Contribution to journalArticle

Suntravat, Montamas ; Langlais, Paul R ; Sánchez, Elda E. ; Nielsen, Vance G. / CatroxMP-II : a heme-modulated fibrinogenolytic metalloproteinase isolated from Crotalus atrox venom. In: BioMetals. 2018 ; Vol. 31, No. 4. pp. 585-593.
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