Cathepsin B and tumor-associated laminin expression in the progression of colorectal adenoma to carcinoma

Ashraf Khan, Murli Krishna, Stephen P. Baker, Barbara F. Banner

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Cathepsin B is a lysosomal cysteine proteinase associated with degradation of laminin. It is increased in colorectal carcinoma (CA). Laminin is a major component of basement membrane involved in cell-matrix interactions and tumor progression. The aim of this study was to correlate cathepsin B and tumor-associated laminin in colorectal adenomas (ADs) with increasing grades of dysplasia and in invasive CAs. Forty-five ADs (8 tubular, 16 tubulovillous, 21 villous), 13 adenomas with high-grade dysplasia/carcinoma in situ (AHDs), and 17 invasive CAs were immunostained with polyclonal antibodies to cathepsin B and laminin. Statistical analysis was performed using exact linear by linear association test and Spearman rank correlation coefficient. Cathepsin B-positive tumor cells were seen in 30 (67%) ADs, 13 (100%) AHDs, and 17 (100%) CAs. The grade of cathepsin B staining was significantly increased in AHDs and CAs, compared with ADs (P < .0001). Laminin was continuous in all of the ADs and discontinuous or fragmented in the AHDs and CAs (P < .0001). The degree of cathepsin B staining in tumor cells also correlated with breakdown of laminin. Increased cathepsin B expression and decrease in tumor-associated laminin might suggest a mechanism for progression of ADs to CAs.

Original languageEnglish (US)
Pages (from-to)704-708
Number of pages5
JournalModern Pathology
Volume11
Issue number8
StatePublished - Aug 1 1998

Keywords

  • Carcinoma
  • Cathepsin B
  • Colorectal adenoma
  • Laminin
  • Progression

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Khan, A., Krishna, M., Baker, S. P., & Banner, B. F. (1998). Cathepsin B and tumor-associated laminin expression in the progression of colorectal adenoma to carcinoma. Modern Pathology, 11(8), 704-708.