Catechol O-methyltransferase pharmacogenomics: Human liver genotype-phenotype correlation and proximal promoter studies

Jianping Zhang, Yuan Ji, Irene Moon, Linda L. Pelleymounter, Oreste Ezequel Salavaggione, Yanhong Wu, Gregory D. Jenkins, Anthony J. Batzler, Daniel J Schaid, Richard M Weinshilboum

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

OBJECTIVES: Catechol O-methyltransferase (COMT) is expressed as both soluble (S) and membrane-bound (MB) isoforms, with S-COMT predominantly expressed in the liver. A common nonsynonymous single nucleotide polymorphism (SNP), 472G>A (108/158Val>Met, S/MB), has been associated with variation in levels of COMT enzyme activity and thermal stability. We set out to test the hypothesis that additional COMT polymorphisms might also be associated with phenotypic variation. METHODS: We phenotyped 268 liver biopsy samples for S-COMT activity and thermal stability, resequenced a portion of the gene that had not been resequenced earlier, and genotyped DNA from these same samples for 16 COMT polymorphisms. RESULTS: There was a significant association between the two COMT phenotypes and genotype at the codon 108 SNP. A haplotype-based approach was then used to assess the possible association of other polymorphisms with phenotype. Specifically, the codon 108 SNP explained 20.4% of variance in enzyme activity (P<10), and 59% of variance in thermal stability (P<10). Haplotypes that included SNPs at cDNA nucleotides 408 and 472 explained additional variance in enzyme activity (up to 24.4%), and the addition to the haplotype of a SNP at intron 2 (51) explained a total of 27.5% of the variance. However, no SNPs beyond that at the nucleotide 472G>A polymorphism were associated with variation in thermal stability. We also observed a three-fold variation in the ability of reporter gene constructs for 'proximal promoter' haplotypes to drive transcription. CONCLUSION: The common COMT 108Val>Met polymorphism is associated with human liver S-COMT activity and thermal stability, but additional COMT SNPs also contribute to variation in activity.

Original languageEnglish (US)
Pages (from-to)577-587
Number of pages11
JournalPharmacogenetics and Genomics
Volume19
Issue number8
DOIs
StatePublished - Aug 2009

Fingerprint

Catechol O-Methyltransferase
Pharmacogenetics
Genetic Association Studies
Liver
Single Nucleotide Polymorphism
Hot Temperature
Codon
Haplotypes
Phenotype
Membranes
Enzymes
Reporter Genes
Protein Isoforms
Genotype
Biopsy

Keywords

  • Catechol O-methyltransferase
  • Genetic polymorphism
  • Haplotype
  • Single nucleotide polymorphism
  • Thermal stability

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Genetics(clinical)

Cite this

Catechol O-methyltransferase pharmacogenomics : Human liver genotype-phenotype correlation and proximal promoter studies. / Zhang, Jianping; Ji, Yuan; Moon, Irene; Pelleymounter, Linda L.; Ezequel Salavaggione, Oreste; Wu, Yanhong; Jenkins, Gregory D.; Batzler, Anthony J.; Schaid, Daniel J; Weinshilboum, Richard M.

In: Pharmacogenetics and Genomics, Vol. 19, No. 8, 08.2009, p. 577-587.

Research output: Contribution to journalArticle

Zhang, Jianping ; Ji, Yuan ; Moon, Irene ; Pelleymounter, Linda L. ; Ezequel Salavaggione, Oreste ; Wu, Yanhong ; Jenkins, Gregory D. ; Batzler, Anthony J. ; Schaid, Daniel J ; Weinshilboum, Richard M. / Catechol O-methyltransferase pharmacogenomics : Human liver genotype-phenotype correlation and proximal promoter studies. In: Pharmacogenetics and Genomics. 2009 ; Vol. 19, No. 8. pp. 577-587.
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abstract = "OBJECTIVES: Catechol O-methyltransferase (COMT) is expressed as both soluble (S) and membrane-bound (MB) isoforms, with S-COMT predominantly expressed in the liver. A common nonsynonymous single nucleotide polymorphism (SNP), 472G>A (108/158Val>Met, S/MB), has been associated with variation in levels of COMT enzyme activity and thermal stability. We set out to test the hypothesis that additional COMT polymorphisms might also be associated with phenotypic variation. METHODS: We phenotyped 268 liver biopsy samples for S-COMT activity and thermal stability, resequenced a portion of the gene that had not been resequenced earlier, and genotyped DNA from these same samples for 16 COMT polymorphisms. RESULTS: There was a significant association between the two COMT phenotypes and genotype at the codon 108 SNP. A haplotype-based approach was then used to assess the possible association of other polymorphisms with phenotype. Specifically, the codon 108 SNP explained 20.4{\%} of variance in enzyme activity (P<10), and 59{\%} of variance in thermal stability (P<10). Haplotypes that included SNPs at cDNA nucleotides 408 and 472 explained additional variance in enzyme activity (up to 24.4{\%}), and the addition to the haplotype of a SNP at intron 2 (51) explained a total of 27.5{\%} of the variance. However, no SNPs beyond that at the nucleotide 472G>A polymorphism were associated with variation in thermal stability. We also observed a three-fold variation in the ability of reporter gene constructs for 'proximal promoter' haplotypes to drive transcription. CONCLUSION: The common COMT 108Val>Met polymorphism is associated with human liver S-COMT activity and thermal stability, but additional COMT SNPs also contribute to variation in activity.",
keywords = "Catechol O-methyltransferase, Genetic polymorphism, Haplotype, Single nucleotide polymorphism, Thermal stability",
author = "Jianping Zhang and Yuan Ji and Irene Moon and Pelleymounter, {Linda L.} and {Ezequel Salavaggione}, Oreste and Yanhong Wu and Jenkins, {Gregory D.} and Batzler, {Anthony J.} and Schaid, {Daniel J} and Weinshilboum, {Richard M}",
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T1 - Catechol O-methyltransferase pharmacogenomics

T2 - Human liver genotype-phenotype correlation and proximal promoter studies

AU - Zhang, Jianping

AU - Ji, Yuan

AU - Moon, Irene

AU - Pelleymounter, Linda L.

AU - Ezequel Salavaggione, Oreste

AU - Wu, Yanhong

AU - Jenkins, Gregory D.

AU - Batzler, Anthony J.

AU - Schaid, Daniel J

AU - Weinshilboum, Richard M

PY - 2009/8

Y1 - 2009/8

N2 - OBJECTIVES: Catechol O-methyltransferase (COMT) is expressed as both soluble (S) and membrane-bound (MB) isoforms, with S-COMT predominantly expressed in the liver. A common nonsynonymous single nucleotide polymorphism (SNP), 472G>A (108/158Val>Met, S/MB), has been associated with variation in levels of COMT enzyme activity and thermal stability. We set out to test the hypothesis that additional COMT polymorphisms might also be associated with phenotypic variation. METHODS: We phenotyped 268 liver biopsy samples for S-COMT activity and thermal stability, resequenced a portion of the gene that had not been resequenced earlier, and genotyped DNA from these same samples for 16 COMT polymorphisms. RESULTS: There was a significant association between the two COMT phenotypes and genotype at the codon 108 SNP. A haplotype-based approach was then used to assess the possible association of other polymorphisms with phenotype. Specifically, the codon 108 SNP explained 20.4% of variance in enzyme activity (P<10), and 59% of variance in thermal stability (P<10). Haplotypes that included SNPs at cDNA nucleotides 408 and 472 explained additional variance in enzyme activity (up to 24.4%), and the addition to the haplotype of a SNP at intron 2 (51) explained a total of 27.5% of the variance. However, no SNPs beyond that at the nucleotide 472G>A polymorphism were associated with variation in thermal stability. We also observed a three-fold variation in the ability of reporter gene constructs for 'proximal promoter' haplotypes to drive transcription. CONCLUSION: The common COMT 108Val>Met polymorphism is associated with human liver S-COMT activity and thermal stability, but additional COMT SNPs also contribute to variation in activity.

AB - OBJECTIVES: Catechol O-methyltransferase (COMT) is expressed as both soluble (S) and membrane-bound (MB) isoforms, with S-COMT predominantly expressed in the liver. A common nonsynonymous single nucleotide polymorphism (SNP), 472G>A (108/158Val>Met, S/MB), has been associated with variation in levels of COMT enzyme activity and thermal stability. We set out to test the hypothesis that additional COMT polymorphisms might also be associated with phenotypic variation. METHODS: We phenotyped 268 liver biopsy samples for S-COMT activity and thermal stability, resequenced a portion of the gene that had not been resequenced earlier, and genotyped DNA from these same samples for 16 COMT polymorphisms. RESULTS: There was a significant association between the two COMT phenotypes and genotype at the codon 108 SNP. A haplotype-based approach was then used to assess the possible association of other polymorphisms with phenotype. Specifically, the codon 108 SNP explained 20.4% of variance in enzyme activity (P<10), and 59% of variance in thermal stability (P<10). Haplotypes that included SNPs at cDNA nucleotides 408 and 472 explained additional variance in enzyme activity (up to 24.4%), and the addition to the haplotype of a SNP at intron 2 (51) explained a total of 27.5% of the variance. However, no SNPs beyond that at the nucleotide 472G>A polymorphism were associated with variation in thermal stability. We also observed a three-fold variation in the ability of reporter gene constructs for 'proximal promoter' haplotypes to drive transcription. CONCLUSION: The common COMT 108Val>Met polymorphism is associated with human liver S-COMT activity and thermal stability, but additional COMT SNPs also contribute to variation in activity.

KW - Catechol O-methyltransferase

KW - Genetic polymorphism

KW - Haplotype

KW - Single nucleotide polymorphism

KW - Thermal stability

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