Casein kinase II and calcineurin modulate TRPP function and ciliary localization

Jinghua Hu, Young Kyung Bae, Karla M. Knobel, Maureen M. Barr

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Cilia serve as sensory devices in a diversity of organisms and their defects contribute to many human diseases. In primary cilia of kidney cells, the transient receptor potential polycystin (TRPP) channels polycystin-1 (PC-1) and polycystin-2 (PC-2) act as a mechanosensitive channel, with defects resulting in autosomal dominant polycystic kidney disease. In sensory cilia of Caenorhabditis elegans male-specific neurons, the TRPPs LOV-1 and PKD-2 are required for mating behavior. The mechanisms regulating TRPP ciliary localization and function are largely unknown. We identified the regulatory subunit of the serine-threonine casein kinase II (CK2) as a binding partner of LOV-1 and human PC-1. CK2 and the calcineurin phosphatase TAX-6 modulate male mating behavior and PKD-2 ciliary localization. The phospho-defective mutant PKD-2 S534A localizes to cilia, whereas a phospho-mimetic PKD-2 S534D mutant is largely absent from cilia. Calcineurin is required for PKD-2 ciliary localization, but is not essential for ciliary gene expression, ciliogenesis, or localization of cilium structural components. This unanticipated function of calcineurin may be important for regulating ciliary protein localization. A dynamic phosphorylation-dephosphorylation cycle may represent a mechanism for modulating TRPP activity, cellular sensation, and ciliary protein localization.

Original languageEnglish (US)
Pages (from-to)2200-2211
Number of pages12
JournalMolecular Biology of the Cell
Volume17
Issue number5
DOIs
StatePublished - May 2006
Externally publishedYes

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TRPP Cation Channels
Casein Kinase II
Cilia
Calcineurin
Transient Receptor Potential Channels
Autosomal Dominant Polycystic Kidney
Protein-Serine-Threonine Kinases
Essential Genes
Caenorhabditis elegans
Proteins
Phosphorylation
Kidney
Gene Expression
Neurons
Equipment and Supplies

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Casein kinase II and calcineurin modulate TRPP function and ciliary localization. / Hu, Jinghua; Bae, Young Kyung; Knobel, Karla M.; Barr, Maureen M.

In: Molecular Biology of the Cell, Vol. 17, No. 5, 05.2006, p. 2200-2211.

Research output: Contribution to journalArticle

Hu, Jinghua ; Bae, Young Kyung ; Knobel, Karla M. ; Barr, Maureen M. / Casein kinase II and calcineurin modulate TRPP function and ciliary localization. In: Molecular Biology of the Cell. 2006 ; Vol. 17, No. 5. pp. 2200-2211.
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