Case study

Polycystic livers in a transgenic mouse line

Jamie Lovaglio, James E. Artwohl, Christopher J. Ward, Thomas G H Diekwisch, Yoshihiro Ito, Jeffrey D. Fortman

Research output: Contribution to journalArticle

Abstract

Three mice (2 male, 1 female; age, 5 to 16 mo) from a mouse line transgenic for keratin 14 (K14)-driven LacZ expression and on an outbred Crl:CD1(ICR) background, were identified as having distended abdomens and livers that were diffusely enlarged by numerous cysts (diameter, 0.1 to 2.0 cm). Histopathology revealed hepatic cysts lined by biliary type epithelium and mild chronic inflammation, and confirmed the absence of parasites. Among 21 related mice, 5 additional affected mice were identified via laparotomy. Breeding of these 5 mice (after 5 mo of age) did not result in any offspring; the K14 mice with polycystic livers failed to reproduce. Affected male mice had degenerative testicular lesions, and their sperm was immotile. Nonpolycystic K14 control male mice bred well, had no testicular lesions, and had appropriate sperm motility. Genetic analysis did not identify an association of this phenotype with the transgene or insertion site.

Original languageEnglish (US)
Pages (from-to)115-120
Number of pages6
JournalComparative Medicine
Volume64
Issue number2
StatePublished - 2014

Fingerprint

Keratin-14
Liver
Transgenic Mice
genetically modified organisms
case studies
liver
mice
keratin
Cysts
lesions (animal)
Sperm Motility
Transgenes
Abdomen
Laparotomy
Breeding
Spermatozoa
laparotomy
Parasites
Epithelium
sperm motility

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)
  • Medicine(all)

Cite this

Lovaglio, J., Artwohl, J. E., Ward, C. J., Diekwisch, T. G. H., Ito, Y., & Fortman, J. D. (2014). Case study: Polycystic livers in a transgenic mouse line. Comparative Medicine, 64(2), 115-120.

Case study : Polycystic livers in a transgenic mouse line. / Lovaglio, Jamie; Artwohl, James E.; Ward, Christopher J.; Diekwisch, Thomas G H; Ito, Yoshihiro; Fortman, Jeffrey D.

In: Comparative Medicine, Vol. 64, No. 2, 2014, p. 115-120.

Research output: Contribution to journalArticle

Lovaglio, J, Artwohl, JE, Ward, CJ, Diekwisch, TGH, Ito, Y & Fortman, JD 2014, 'Case study: Polycystic livers in a transgenic mouse line', Comparative Medicine, vol. 64, no. 2, pp. 115-120.
Lovaglio J, Artwohl JE, Ward CJ, Diekwisch TGH, Ito Y, Fortman JD. Case study: Polycystic livers in a transgenic mouse line. Comparative Medicine. 2014;64(2):115-120.
Lovaglio, Jamie ; Artwohl, James E. ; Ward, Christopher J. ; Diekwisch, Thomas G H ; Ito, Yoshihiro ; Fortman, Jeffrey D. / Case study : Polycystic livers in a transgenic mouse line. In: Comparative Medicine. 2014 ; Vol. 64, No. 2. pp. 115-120.
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