Carotid repair using autologous adipose-derived endothelial cells

Harald Froehlich, Rajiv Gulati, Barry Boilson, Tyra Witt, Adriana Harbuzariu, Laurel Kleppe, Allan B Dietz, Amir Lerman, Robert D. Simari

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background and Purpose-: Adipose tissue is an abundant source of endothelial cells as well as stem and progenitor cells which can develop an endothelial phenotype. It has been demonstrated that these cells have distinct angiogenic properties in vitro and in vivo. However, whether these cells have the capacity to directly improve large vessel form and function after vascular injury remains unknown. To define whether delivery of adipose-derived endothelial cells (ADECs) would improve healing of injured carotid arteries, a rabbit model of acute arterial injury was used. Methods-: Autologous rabbit ADECs were generated using defined culture conditions. To test the ability of ADECs to enhance carotid artery repair, cells were delivered intraarterially after acute balloon injury. Additional delivery studies were performed after functional selection of cells before delivery. Results-: After rabbit omental fat harvest and digestion, a proliferative, homogenous, and distinctly endothelial population of ADECs was identified. Direct delivery of autologous ADECs resulted in marked reendothelialization 48 hours after acute vascular injury as compared to saline controls (82.2±26.9% versus 4.2±3.0% P<0.001). Delivery of ADECs that were selected for their ability to take up acetylated LDL significantly improved vasoreactivity and decreased intimal formation after vascular injury. Conclusions-: Taken together, these data suggest that ADECs represent an autologous source of proliferative endothelial cells, which demonstrate the capacity to rapidly improve reendothelialization, improve vascular reactivity, and decrease intimal formation in a carotid artery injury model.

Original languageEnglish (US)
Pages (from-to)1886-1891
Number of pages6
JournalStroke
Volume40
Issue number5
DOIs
StatePublished - May 1 2009

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Endothelial Cells
Vascular System Injuries
Tunica Intima
Rabbits
Carotid Arteries
Stem Cells
Carotid Artery Injuries
Wounds and Injuries
Blood Vessels
Adipose Tissue
Digestion
Fats
Phenotype
Population

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing
  • Medicine(all)

Cite this

Froehlich, H., Gulati, R., Boilson, B., Witt, T., Harbuzariu, A., Kleppe, L., ... Simari, R. D. (2009). Carotid repair using autologous adipose-derived endothelial cells. Stroke, 40(5), 1886-1891. https://doi.org/10.1161/STROKEAHA.108.539932

Carotid repair using autologous adipose-derived endothelial cells. / Froehlich, Harald; Gulati, Rajiv; Boilson, Barry; Witt, Tyra; Harbuzariu, Adriana; Kleppe, Laurel; Dietz, Allan B; Lerman, Amir; Simari, Robert D.

In: Stroke, Vol. 40, No. 5, 01.05.2009, p. 1886-1891.

Research output: Contribution to journalArticle

Froehlich, H, Gulati, R, Boilson, B, Witt, T, Harbuzariu, A, Kleppe, L, Dietz, AB, Lerman, A & Simari, RD 2009, 'Carotid repair using autologous adipose-derived endothelial cells', Stroke, vol. 40, no. 5, pp. 1886-1891. https://doi.org/10.1161/STROKEAHA.108.539932
Froehlich H, Gulati R, Boilson B, Witt T, Harbuzariu A, Kleppe L et al. Carotid repair using autologous adipose-derived endothelial cells. Stroke. 2009 May 1;40(5):1886-1891. https://doi.org/10.1161/STROKEAHA.108.539932
Froehlich, Harald ; Gulati, Rajiv ; Boilson, Barry ; Witt, Tyra ; Harbuzariu, Adriana ; Kleppe, Laurel ; Dietz, Allan B ; Lerman, Amir ; Simari, Robert D. / Carotid repair using autologous adipose-derived endothelial cells. In: Stroke. 2009 ; Vol. 40, No. 5. pp. 1886-1891.
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