Cardiotrophin-1 stimulates endothelin-1 via gp130 in vascular endothelial cells

Michihisa Jougasaki, Amy M. Larsen, Alessandro Cataliotti, David C. Christiansen, John C Jr. Burnett

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Endothelin-1 (ET-1) is a vasoconstricting and mitogenic peptide released from vascular endothelial cells under normal and pathophysiological conditions, and synthesis and secretion of ET-1 are stimulated by cytokines. Cardiotrophin-1 (CT-1) is a new member of the interleukin-6-type cytokines that induce biological actions through the glycoprotein (gp) 130. The present study was designed to determine the presence of CT-1 and the gp130 cytokine system in vascular endothelial cells and to investigate whether CT-1 stimulates synthesis and secretion of ET-1 in the vascular endothelial cells. We first sought to determine gene expression and immunoreactivity of CT-1, gp130 and ET-1 in cultured canine aortic endothelial cells (CAECs) using Northern blot analysis and immunocytochemistry, which revealed the presence of CT-1 and gp130 together with ET-1 in CAECs. CT-1 increased ET-1 gene expression in CAECs, and stimulated ET-1 secretion from CAECs in a dose-dependent manner. Furthermore, inhibition of gp130 by monoclonal antibody attenuated ET-1 secretion from CAECs, suggesting that actions of CT-1 on the secretion of ET-1 are mediated through gp130 receptor system. The present study, therefore, reports the presence of CT-1 and gp130 in vascular endothelial cells and mechanisms of secretion of ET-1 related to this cytokine system.

Original languageEnglish (US)
Pages (from-to)1441-1447
Number of pages7
JournalPeptides
Volume23
Issue number8
DOIs
StatePublished - Aug 2002

Fingerprint

Endothelial cells
Endothelin-1
Endothelial Cells
Canidae
Cytokines
Gene expression
cardiotrophin 1
Gene Expression
Northern Blotting
Interleukin-6
Glycoproteins
Immunohistochemistry
Monoclonal Antibodies
Peptides

Keywords

  • Endothelium
  • Hormone
  • Immunocytochemistry
  • Peptides
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Cardiotrophin-1 stimulates endothelin-1 via gp130 in vascular endothelial cells. / Jougasaki, Michihisa; Larsen, Amy M.; Cataliotti, Alessandro; Christiansen, David C.; Burnett, John C Jr.

In: Peptides, Vol. 23, No. 8, 08.2002, p. 1441-1447.

Research output: Contribution to journalArticle

Jougasaki, M, Larsen, AM, Cataliotti, A, Christiansen, DC & Burnett, JCJ 2002, 'Cardiotrophin-1 stimulates endothelin-1 via gp130 in vascular endothelial cells', Peptides, vol. 23, no. 8, pp. 1441-1447. https://doi.org/10.1016/S0196-9781(02)00078-5
Jougasaki, Michihisa ; Larsen, Amy M. ; Cataliotti, Alessandro ; Christiansen, David C. ; Burnett, John C Jr. / Cardiotrophin-1 stimulates endothelin-1 via gp130 in vascular endothelial cells. In: Peptides. 2002 ; Vol. 23, No. 8. pp. 1441-1447.
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