Cardiac extracellular matrix proteomics

Challenges, techniques, and clinical implications

Chia Wei Chang, Ailsa J. Dalgliesh, Javier E. López, Leigh Griffiths

Research output: Contribution to journalReview article

12 Citations (Scopus)

Abstract

Extracellular matrix (ECM) has emerged as a dynamic tissue component, providing not only structural support, but also functionally participating in a wide range of signaling events during development, injury, and disease remodeling. Investigation of dynamic changes in cardiac ECM proteome is challenging due to the relative insolubility of ECM proteins, which results from their macromolecular nature, extensive post-translational modification (PTM), and tendency to form protein complexes. Finally, the relative abundance of cellular and mitochondrial proteins in cardiac tissue further complicates cardiac ECM proteomic approaches. Recent developments of various techniques to enrich and analyze ECM proteins are playing a major role in overcoming these challenges. Application of cardiac ECM proteomics in disease tissues can further provide spatial and temporal information relevant to disease diagnosis, prognosis, treatment, and engineering of therapeutic candidates for cardiac repair and regeneration.

Original languageEnglish (US)
Pages (from-to)39-50
Number of pages12
JournalProteomics - Clinical Applications
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Proteomics
Extracellular Matrix
Extracellular Matrix Proteins
Tissue
Mitochondrial Proteins
Proteome
Repair
Post Translational Protein Processing
Solubility
Regeneration
Wounds and Injuries
Proteins
Therapeutics

Keywords

  • Cardiac diseases
  • Decellularization
  • Extracellular matrix biomarker
  • Extracellular matrix remodeling
  • Extracellular proteomics

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Cardiac extracellular matrix proteomics : Challenges, techniques, and clinical implications. / Chang, Chia Wei; Dalgliesh, Ailsa J.; López, Javier E.; Griffiths, Leigh.

In: Proteomics - Clinical Applications, Vol. 10, No. 1, 01.01.2016, p. 39-50.

Research output: Contribution to journalReview article

Chang, Chia Wei ; Dalgliesh, Ailsa J. ; López, Javier E. ; Griffiths, Leigh. / Cardiac extracellular matrix proteomics : Challenges, techniques, and clinical implications. In: Proteomics - Clinical Applications. 2016 ; Vol. 10, No. 1. pp. 39-50.
@article{cc1d8e28fe5b44bb84e7870dfafa7ed5,
title = "Cardiac extracellular matrix proteomics: Challenges, techniques, and clinical implications",
abstract = "Extracellular matrix (ECM) has emerged as a dynamic tissue component, providing not only structural support, but also functionally participating in a wide range of signaling events during development, injury, and disease remodeling. Investigation of dynamic changes in cardiac ECM proteome is challenging due to the relative insolubility of ECM proteins, which results from their macromolecular nature, extensive post-translational modification (PTM), and tendency to form protein complexes. Finally, the relative abundance of cellular and mitochondrial proteins in cardiac tissue further complicates cardiac ECM proteomic approaches. Recent developments of various techniques to enrich and analyze ECM proteins are playing a major role in overcoming these challenges. Application of cardiac ECM proteomics in disease tissues can further provide spatial and temporal information relevant to disease diagnosis, prognosis, treatment, and engineering of therapeutic candidates for cardiac repair and regeneration.",
keywords = "Cardiac diseases, Decellularization, Extracellular matrix biomarker, Extracellular matrix remodeling, Extracellular proteomics",
author = "Chang, {Chia Wei} and Dalgliesh, {Ailsa J.} and L{\'o}pez, {Javier E.} and Leigh Griffiths",
year = "2016",
month = "1",
day = "1",
doi = "10.1002/prca.201500030",
language = "English (US)",
volume = "10",
pages = "39--50",
journal = "Proteomics - Clinical Applications",
issn = "1862-8346",
publisher = "Wiley-VCH Verlag",
number = "1",

}

TY - JOUR

T1 - Cardiac extracellular matrix proteomics

T2 - Challenges, techniques, and clinical implications

AU - Chang, Chia Wei

AU - Dalgliesh, Ailsa J.

AU - López, Javier E.

AU - Griffiths, Leigh

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Extracellular matrix (ECM) has emerged as a dynamic tissue component, providing not only structural support, but also functionally participating in a wide range of signaling events during development, injury, and disease remodeling. Investigation of dynamic changes in cardiac ECM proteome is challenging due to the relative insolubility of ECM proteins, which results from their macromolecular nature, extensive post-translational modification (PTM), and tendency to form protein complexes. Finally, the relative abundance of cellular and mitochondrial proteins in cardiac tissue further complicates cardiac ECM proteomic approaches. Recent developments of various techniques to enrich and analyze ECM proteins are playing a major role in overcoming these challenges. Application of cardiac ECM proteomics in disease tissues can further provide spatial and temporal information relevant to disease diagnosis, prognosis, treatment, and engineering of therapeutic candidates for cardiac repair and regeneration.

AB - Extracellular matrix (ECM) has emerged as a dynamic tissue component, providing not only structural support, but also functionally participating in a wide range of signaling events during development, injury, and disease remodeling. Investigation of dynamic changes in cardiac ECM proteome is challenging due to the relative insolubility of ECM proteins, which results from their macromolecular nature, extensive post-translational modification (PTM), and tendency to form protein complexes. Finally, the relative abundance of cellular and mitochondrial proteins in cardiac tissue further complicates cardiac ECM proteomic approaches. Recent developments of various techniques to enrich and analyze ECM proteins are playing a major role in overcoming these challenges. Application of cardiac ECM proteomics in disease tissues can further provide spatial and temporal information relevant to disease diagnosis, prognosis, treatment, and engineering of therapeutic candidates for cardiac repair and regeneration.

KW - Cardiac diseases

KW - Decellularization

KW - Extracellular matrix biomarker

KW - Extracellular matrix remodeling

KW - Extracellular proteomics

UR - http://www.scopus.com/inward/record.url?scp=84953345265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84953345265&partnerID=8YFLogxK

U2 - 10.1002/prca.201500030

DO - 10.1002/prca.201500030

M3 - Review article

VL - 10

SP - 39

EP - 50

JO - Proteomics - Clinical Applications

JF - Proteomics - Clinical Applications

SN - 1862-8346

IS - 1

ER -