Carboxypeptidase M: A biomarker for the discrimination of well-differentiated liposarcoma from lipoma

Michele R. Erickson-Johnson, Amber R. Seys, Christopher W. Roth, Allison A. King, Rachael L. Hulshizer, Xiaoke Wang, Yan W. Asmann, Ricardo V. Lloyd, Eapen K. Jacob, Andre M. Oliveira

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

The discrimination between well-differentiated liposarcomas/atypical lipomatous tumors and lipomas can be diagnostically challenging at the histological level. However, cytogenetic identification of ring and giant rod chromosomes supports the diagnosis of well-differentiated liposarcoma/atypical lipomatous tumor. These abnormal chromosomes are mainly composed of amplified genomic sequences derived from chromosome 12q13-15, and contain several genes, including MDM2, CDK4 (SAS), TSPAN31, HMGA2, and others. MDM2 is consistently amplified in well-differentiated liposarcomas/atypical lipomatous tumors, and up to 25% in other sarcomas. As part of a large genomic study of lipomatous neoplasms, we initially found CPM to be consistently amplified in well-differentiated liposarcomas/atypical lipomatous tumors. To further explore this initial finding, we investigated the copy number status of MDM2 and CPM by fluorescent in situ hybridization (FISH) on a series of 138 tumors and 17 normal tissues, including 32 well-differentiated liposarcoma/atypical lipomatous tumors, 63 lipomas, 11 pleomorphic lipomas, 2 lipoblastomas, 30 other tumors and 17 normal fat samples. All 32 well-differentiated liposarcoma/atypical lipomatous tumors showed amplification of MDM2 and CPM, usually 20 copies per cell. The other tumors lacked MDM2 and/or CPM amplification. Chromogenic in situ hybridization confirmed the above results on a subset of these tumors (n27). These findings suggest that identification of CPM amplification could be used as an alternative diagnostic tool for the diagnosis of well-differentiated liposarcoma/atypical lipomatous tumors.

Original languageEnglish (US)
Pages (from-to)1541-1547
Number of pages7
JournalModern Pathology
Volume22
Issue number12
DOIs
StatePublished - Dec 1 2009

    Fingerprint

Keywords

  • CPM
  • FISH
  • Lipoma
  • MDM2
  • Well-differentiated liposarcoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Erickson-Johnson, M. R., Seys, A. R., Roth, C. W., King, A. A., Hulshizer, R. L., Wang, X., Asmann, Y. W., Lloyd, R. V., Jacob, E. K., & Oliveira, A. M. (2009). Carboxypeptidase M: A biomarker for the discrimination of well-differentiated liposarcoma from lipoma. Modern Pathology, 22(12), 1541-1547. https://doi.org/10.1038/modpathol.2009.149