Capillary-like network formation by human amniotic fluid-derived stem cells within fibrin/poly(ethylene glycol) hydrogels

Omar M. Benavides, Joseph P. Quinn, Seokwon Pok, Jennifer Petsche Connell, Rodrigo Ruano, Jeffrey G. Jacot

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A major limitation in tissue engineering strategies for congenital birth defects is the inability to provide a significant source of oxygen, nutrient, and waste transport in an avascular scaffold. Successful vascularization requires a reliable method to generate vascular cells and a scaffold capable of supporting vessel formation. The broad potential for differentiation, high proliferation rates, and autologous availability for neonatal surgeries make amniotic fluid-derived stem cells (AFSC) well suited for regenerative medicine strategies. AFSC-derived endothelial cells (AFSC-EC) express key proteins and functional phenotypes associated with endothelial cells. Fibrin-based hydrogels were shown to stimulate AFSC-derived network formation in vitro but were limited by rapid degradation. Incorporation of poly(ethylene glycol) (PEG) provided mechanical stability (65%±9% weight retention vs. 0% for fibrin-only at day 14) while retaining key benefits of fibrin-based scaffolds - quick formation (10±3 s), biocompatibility (88%±5% viability), and vasculogenic stimulation. To determine the feasibility of AFSC-derived microvasculature, we compared AFSC-EC as a vascular cell source and AFSC as a perivascular cell source to established sources of these cell types - human umbilical vein endothelial cells (HUVEC) and mesenchymal stem cells (MSC), respectively. Cocultures were seeded at a 4:1 endothelial-to-perivascular cell ratio, and gels were incubated at 37°C for 2 weeks. Mechanical testing was performed using a stress-controlled rheometer (G′=95±10 Pa), and cell-seeded hydrogels were assessed based on morphology. Network formation was analyzed based on key parameters such as vessel thickness, length, and area, as well as the degree of branching. There was no statistical difference between individual cultures of AFSC-EC and HUVEC in regard to these parameters, suggesting the vasculogenic potential of AFSC-EC; however, the development of robust vessels required the presence of both an endothelial and a perivascular cell source and was seen in AFSC cocultures (70%±20% vessel length, 90%±10% vessel area, and 105%±10% vessel thickness compared to HUVEC/MSC). At a fixed seeding density, the coculture of AFSC with AFSC-EC resulted in a synergistic effect on network parameters similar to MSC (150% vessel length, 147% vessel area, 150% vessel thickness, and 155% branching). These results suggest that AFSC-EC and AFSC have significant vasculogenic and perivasculogenic potential, respectively, and are suited for in vivo evaluation.

Original languageEnglish (US)
Pages (from-to)1185-1194
Number of pages10
JournalTissue Engineering - Part A
Volume21
Issue number7-8
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Hydrogels
Ethylene Glycol
Amniotic Fluid
Stem cells
Fibrin
Polyethylene glycols
Stem Cells
Fluids
Endothelial cells
Human Umbilical Vein Endothelial Cells
Coculture Techniques
Mesenchymal Stromal Cells
Blood Vessels
Scaffolds (biology)
Endothelial Cells
Regenerative Medicine
Tissue Engineering
Microvessels
Individuality
Mechanical stability

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Capillary-like network formation by human amniotic fluid-derived stem cells within fibrin/poly(ethylene glycol) hydrogels. / Benavides, Omar M.; Quinn, Joseph P.; Pok, Seokwon; Petsche Connell, Jennifer; Ruano, Rodrigo; Jacot, Jeffrey G.

In: Tissue Engineering - Part A, Vol. 21, No. 7-8, 01.01.2015, p. 1185-1194.

Research output: Contribution to journalArticle

Benavides, Omar M. ; Quinn, Joseph P. ; Pok, Seokwon ; Petsche Connell, Jennifer ; Ruano, Rodrigo ; Jacot, Jeffrey G. / Capillary-like network formation by human amniotic fluid-derived stem cells within fibrin/poly(ethylene glycol) hydrogels. In: Tissue Engineering - Part A. 2015 ; Vol. 21, No. 7-8. pp. 1185-1194.
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