Capillary electrophoretic assay for nitrate levels in the vitreous of proliferative diabetic retinopathy

Leyi Gao, Jose S. Pulido, R. Mark Hatfield, Robert F. Dundervill, Colin A. McCannel, Scott A. Shippy

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The determination of nitric oxide (NO) in human vitreous samples is complicated by the relatively short half-life of the analyte and the viscous, high salt and protein biological matrix. In this work, we developed a fast (<5 min) and useful CE method to determine the stable metabolite, nitrate, from vitreous samples. This proposed method has been successfully applied to determine the nitrate levels from the vitreous humor of patients undergoing vitrectomy for a variety of conditions. A statistically significant increase (P = 0.000001) of the mean level of nitrate was observed in vitreous humor of patients with proliferative diabetic retinopathy (41.17 ± 4.09 μM, n = 27) versus controls (15.22 ± 0.86 μM, n = 35). The elevated levels of nitrate in the vitreous of patients known to have diabetic retinopathy suggests that NO is involved with the pathology of this disease.

Original languageEnglish (US)
Pages (from-to)300-304
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume847
Issue number2
DOIs
StatePublished - Mar 1 2007

Keywords

  • Capillary electrophoresis
  • Clinical samples
  • Nitric oxide
  • PDR

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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