CAP survey of parathyroid hormone assays

G. G. Klee, J. Shikegawa, T. D. Trainer

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Parathyroid hormone (PTH) analysis was recently added to the College of American Pathologists ligand assay survey. Synthetic whole-molecule PTH was used as the test hormone. This material was found to be stable in lyophilized serum for at least 10.7 days at 35°C and 3.9 days at 45° C. The survey results showed wide ranges of answers yielding within-assay coefficients of variation from 12% to 93%. The median percentage of the spiked hormone that was recovered by the various assay methods ranged from 24% to 390%. Separation of the spiked and endogenous measured PTH concentrations ranged from a twofold separation to an approximate 50% overlap of results. The survey may not be a valid indicator of PTH assay efficacy since whole-molecule human PTH may not be the most clinically important form of circulating PTH; however, the survey shows that there is a large heterogeneity in commercial PTH assays.

Original languageEnglish (US)
Pages (from-to)588-591
Number of pages4
JournalArchives of Pathology and Laboratory Medicine
Volume110
Issue number7
StatePublished - 1986

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Parathyroid Hormone
Hormones
Surveys and Questionnaires
Ligands
Serum

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Klee, G. G., Shikegawa, J., & Trainer, T. D. (1986). CAP survey of parathyroid hormone assays. Archives of Pathology and Laboratory Medicine, 110(7), 588-591.

CAP survey of parathyroid hormone assays. / Klee, G. G.; Shikegawa, J.; Trainer, T. D.

In: Archives of Pathology and Laboratory Medicine, Vol. 110, No. 7, 1986, p. 588-591.

Research output: Contribution to journalArticle

Klee, GG, Shikegawa, J & Trainer, TD 1986, 'CAP survey of parathyroid hormone assays', Archives of Pathology and Laboratory Medicine, vol. 110, no. 7, pp. 588-591.
Klee GG, Shikegawa J, Trainer TD. CAP survey of parathyroid hormone assays. Archives of Pathology and Laboratory Medicine. 1986;110(7):588-591.
Klee, G. G. ; Shikegawa, J. ; Trainer, T. D. / CAP survey of parathyroid hormone assays. In: Archives of Pathology and Laboratory Medicine. 1986 ; Vol. 110, No. 7. pp. 588-591.
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