CAP survey of parathyroid hormone assays

G. G. Klee, J. Shikegawa, T. D. Trainer

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Parathyroid hormone (PTH) analysis was recently added to the College of American Pathologists ligand assay survey. Synthetic whole-molecule PTH was used as the test hormone. This material was found to be stable in lyophilized serum for at least 10.7 days at 35°C and 3.9 days at 45° C. The survey results showed wide ranges of answers yielding within-assay coefficients of variation from 12% to 93%. The median percentage of the spiked hormone that was recovered by the various assay methods ranged from 24% to 390%. Separation of the spiked and endogenous measured PTH concentrations ranged from a twofold separation to an approximate 50% overlap of results. The survey may not be a valid indicator of PTH assay efficacy since whole-molecule human PTH may not be the most clinically important form of circulating PTH; however, the survey shows that there is a large heterogeneity in commercial PTH assays.

Original languageEnglish (US)
Pages (from-to)588-591
Number of pages4
JournalArchives of Pathology and Laboratory Medicine
Volume110
Issue number7
StatePublished - 1986

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

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    Klee, G. G., Shikegawa, J., & Trainer, T. D. (1986). CAP survey of parathyroid hormone assays. Archives of Pathology and Laboratory Medicine, 110(7), 588-591.