Canine distemper virus uses both the anterograde and the hematogenous pathway for neuroinvasion

Penny A. Rudd, Roberto Cattaneo, Veronika Von Messling

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Canine distemper virus (CDV), a member of the Morbillivirus genus that also includes measles virus, frequently causes neurologic complications, but the routes and timing of CDV invasion of the central nervous system (CNS) are poorly understood. To characterize these events, we cloned and sequenced the genome of a neurovirulent CDV (strain A75/17) and produced an infectious cDNA that expresses the green fluorescent protein. This virus fully retained its virulence in ferrets: the course and signs of disease were equivalent to those of the parental isolate. We observed CNS invasion through two distinct pathways: anterogradely via the olfactory nerve and hematogenously through the choroid plexus and cerebral blood vessels. CNS invasion only occurred after massive infection of the lymphatic system and spread to the epithelial cells throughout the body. While at early time points, mostly immune and endothelial cells were infected, the virus later spread to glial cells and neurons. Together, the results suggest similarities in the timing, target cells, and CNS invasion routes of CDV, members of the Morbillivirus genus, and even other neurovirulent paramyxoviruses like Nipah and mumps viruses.

Original languageEnglish (US)
Pages (from-to)9361-9370
Number of pages10
JournalJournal of Virology
Volume80
Issue number19
DOIs
StatePublished - Oct 2006

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Canine Distemper Virus
Canine distemper virus
central nervous system
Morbillivirus
Central Nervous System
Mumps virus
Nipah Virus
Nipah virus
lymphatic system
Olfactory Nerve
Measles virus
Viruses
Respirovirus
choroid plexus
Lymphatic System
viruses
Ferrets
Choroid Plexus
ferrets
neuroglia

ASJC Scopus subject areas

  • Immunology

Cite this

Canine distemper virus uses both the anterograde and the hematogenous pathway for neuroinvasion. / Rudd, Penny A.; Cattaneo, Roberto; Von Messling, Veronika.

In: Journal of Virology, Vol. 80, No. 19, 10.2006, p. 9361-9370.

Research output: Contribution to journalArticle

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