CaM kinase II isoforms are expressed at lower levels in brains of shiverer mutants

Christopher T. Suznovich, Rongsun Pu, Allan J. Bieber

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the mammalian brain, the hippocampus is involved in memory formation and storage and has an enriched level of Ca2+/calmodulin-dependent protein kinase type II (CaM kinase II). CaM kinase II has a number of downstream targets and is shown to play a role in memory development, axonal transport, and signaling across the synapse. The shiverer mutant mouse is a knockout lacking myelin basic protein. As a result, the axons of the central nervous system (CNS) of the shiverer have no or very thin myelin sheath, neurons in their CNS have distorted shapes, and synaptic signaling is impaired. shiverer mice develop symptoms similar to those experienced by patients with multiple sclerosis. In this study, proteins from the hippocampus, cerebellum, pons, medulla, and olfactory bulbs of shiverer and wild-type mice were extracted. Western blot analysis was used to compare the expression levels of CaM kinase II in these regions of the two types of mice. Analysis shows that at least two (50 and 58-59 kDa) of the four CaM kinase II isoforms are expressed in the brain, with one isoform (50 kDa) expressed in all regions examined. shiverer brain contains a decreased level of the two isoforms of CaM kinase II, an indication that the cognitive function of these mice might also be impaired.

Original languageEnglish (US)
Pages (from-to)297-305
Number of pages9
JournalJournal of Molecular Neuroscience
Volume31
Issue number3
StatePublished - Mar 2007

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Brain
Protein Isoforms
Neurology
Hippocampus
Central Nervous System
Data storage equipment
Axonal Transport
Myelin Basic Protein
Pons
Olfactory Bulb
Myelin Sheath
Synapses
Cerebellum
Cognition
Neurons
Multiple Sclerosis
Axons
Western Blotting
Proteins

Keywords

  • CaM kinase II
  • Multiple sclerosis
  • Shiverer mouse

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Neuroscience(all)

Cite this

CaM kinase II isoforms are expressed at lower levels in brains of shiverer mutants. / Suznovich, Christopher T.; Pu, Rongsun; Bieber, Allan J.

In: Journal of Molecular Neuroscience, Vol. 31, No. 3, 03.2007, p. 297-305.

Research output: Contribution to journalArticle

Suznovich, Christopher T. ; Pu, Rongsun ; Bieber, Allan J. / CaM kinase II isoforms are expressed at lower levels in brains of shiverer mutants. In: Journal of Molecular Neuroscience. 2007 ; Vol. 31, No. 3. pp. 297-305.
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