TY - JOUR
T1 - Calmodulin wraps around its binding domain in the plasma membrane Ca 2+ pump anchored by a novel 18-1 motif
AU - Juranic, Nenad
AU - Atanasova, Elena
AU - Filoteo, Adelaida G.
AU - Macura, Slobodan
AU - Prendergast, Franklyn G.
AU - Penniston, John T.
AU - Strehler, Emanuel E.
PY - 2010/2/5
Y1 - 2010/2/5
N2 - Using solution NMR spectroscopy, we obtained the structure of Ca 2+-calmodulin (holoCaM) in complex with peptide C28 from the binding domain of the plasma membrane Ca2+-ATPase (PMCA) pump isoform 4b. This provides the first atomic resolution insight into the binding mode of holoCaM to the full-length binding domain of PMCA. Structural comparison of the previously determined holoCaM·C20 complex with this holoCaM·C28 complex supports the idea that the initial binding step is represented by (holoCaM·C20) and the final bound complex by (holoCaM·C28). This affirms the existing multi-step kinetic model of PMCA4b activation by CaM. The complex exhibits a new binding motif in which holoCaM is wrapped around helical C28 peptide using two anchoring residues from the peptide at relative positions 18 and 1. The anchors correspond to Phe-1110 and Trp-1093, respectively, in full-length PMCA4b, and the peptide and CaM are oriented in an anti-parallel manner. This is a greater sequence distance between anchors than in any of the known holoCaM complexes with a helical peptide. Analysis of the geometry of holoCaM-peptide binding for the cases where the target peptide adopts an αD-helix with its anchors buried in the main hydrophobic pockets of the two CaM lobes establishes that only relative sequential positions of 10, 14, 17, and 18 are allowed for the second anchor.
AB - Using solution NMR spectroscopy, we obtained the structure of Ca 2+-calmodulin (holoCaM) in complex with peptide C28 from the binding domain of the plasma membrane Ca2+-ATPase (PMCA) pump isoform 4b. This provides the first atomic resolution insight into the binding mode of holoCaM to the full-length binding domain of PMCA. Structural comparison of the previously determined holoCaM·C20 complex with this holoCaM·C28 complex supports the idea that the initial binding step is represented by (holoCaM·C20) and the final bound complex by (holoCaM·C28). This affirms the existing multi-step kinetic model of PMCA4b activation by CaM. The complex exhibits a new binding motif in which holoCaM is wrapped around helical C28 peptide using two anchoring residues from the peptide at relative positions 18 and 1. The anchors correspond to Phe-1110 and Trp-1093, respectively, in full-length PMCA4b, and the peptide and CaM are oriented in an anti-parallel manner. This is a greater sequence distance between anchors than in any of the known holoCaM complexes with a helical peptide. Analysis of the geometry of holoCaM-peptide binding for the cases where the target peptide adopts an αD-helix with its anchors buried in the main hydrophobic pockets of the two CaM lobes establishes that only relative sequential positions of 10, 14, 17, and 18 are allowed for the second anchor.
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U2 - 10.1074/jbc.M109.060491
DO - 10.1074/jbc.M109.060491
M3 - Article
C2 - 19996092
AN - SCOPUS:77950473953
SN - 0021-9258
VL - 285
SP - 4015
EP - 4024
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 6
ER -