Calmodulin wraps around its binding domain in the plasma membrane Ca 2+ pump anchored by a novel 18-1 motif

Nenad Juranic, Elena Atanasova, Adelaida G. Filoteo, Slobodan I Macura, Franklyn G. Prendergast, John T. Penniston, Emanuel E. Strehler

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Using solution NMR spectroscopy, we obtained the structure of Ca 2+-calmodulin (holoCaM) in complex with peptide C28 from the binding domain of the plasma membrane Ca2+-ATPase (PMCA) pump isoform 4b. This provides the first atomic resolution insight into the binding mode of holoCaM to the full-length binding domain of PMCA. Structural comparison of the previously determined holoCaM·C20 complex with this holoCaM·C28 complex supports the idea that the initial binding step is represented by (holoCaM·C20) and the final bound complex by (holoCaM·C28). This affirms the existing multi-step kinetic model of PMCA4b activation by CaM. The complex exhibits a new binding motif in which holoCaM is wrapped around helical C28 peptide using two anchoring residues from the peptide at relative positions 18 and 1. The anchors correspond to Phe-1110 and Trp-1093, respectively, in full-length PMCA4b, and the peptide and CaM are oriented in an anti-parallel manner. This is a greater sequence distance between anchors than in any of the known holoCaM complexes with a helical peptide. Analysis of the geometry of holoCaM-peptide binding for the cases where the target peptide adopts an αD-helix with its anchors buried in the main hydrophobic pockets of the two CaM lobes establishes that only relative sequential positions of 10, 14, 17, and 18 are allowed for the second anchor.

Original languageEnglish (US)
Pages (from-to)4015-4024
Number of pages10
JournalJournal of Biological Chemistry
Volume285
Issue number6
DOIs
StatePublished - Feb 5 2010

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Calmodulin
Cell membranes
Cell Membrane
Pumps
Peptides
Anchors
Calcium-Transporting ATPases
Nuclear magnetic resonance spectroscopy
Protein Isoforms
Magnetic Resonance Spectroscopy
Chemical activation
Kinetics
Geometry

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Juranic, N., Atanasova, E., Filoteo, A. G., Macura, S. I., Prendergast, F. G., Penniston, J. T., & Strehler, E. E. (2010). Calmodulin wraps around its binding domain in the plasma membrane Ca 2+ pump anchored by a novel 18-1 motif. Journal of Biological Chemistry, 285(6), 4015-4024. https://doi.org/10.1074/jbc.M109.060491

Calmodulin wraps around its binding domain in the plasma membrane Ca 2+ pump anchored by a novel 18-1 motif. / Juranic, Nenad; Atanasova, Elena; Filoteo, Adelaida G.; Macura, Slobodan I; Prendergast, Franklyn G.; Penniston, John T.; Strehler, Emanuel E.

In: Journal of Biological Chemistry, Vol. 285, No. 6, 05.02.2010, p. 4015-4024.

Research output: Contribution to journalArticle

Juranic, N, Atanasova, E, Filoteo, AG, Macura, SI, Prendergast, FG, Penniston, JT & Strehler, EE 2010, 'Calmodulin wraps around its binding domain in the plasma membrane Ca 2+ pump anchored by a novel 18-1 motif', Journal of Biological Chemistry, vol. 285, no. 6, pp. 4015-4024. https://doi.org/10.1074/jbc.M109.060491
Juranic, Nenad ; Atanasova, Elena ; Filoteo, Adelaida G. ; Macura, Slobodan I ; Prendergast, Franklyn G. ; Penniston, John T. ; Strehler, Emanuel E. / Calmodulin wraps around its binding domain in the plasma membrane Ca 2+ pump anchored by a novel 18-1 motif. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 6. pp. 4015-4024.
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